摘要
目的探讨龟鹿二仙胶醇提物含药血清对体外培养破骨细胞骨吸收功能的影响。方法体外诱导破骨前体细胞系RAW264.7向破骨细胞分化,分别用龟鹿二仙胶醇提物含药血清和生理盐水血清干预,实时荧光定量PCR检测龟鹿二仙胶汤及其拆方含药血清对破骨细胞抗酒石酸酸性磷酸酶(TRAP)、组织蛋白酶K(Cts K)、降钙素受体(CTR)mRNA表达。结果与生理盐水对照组相比,龟鹿二仙胶醇提物含药血清可降低破骨细胞骨Cts K(P<0.01)、TRAP(P<0.01)mRNA的表达,提高CTR(P<0.01)mRNA的表达。结论龟鹿二仙胶及其拆方醇提物含药血清可降低破骨细胞骨吸收活性。
OBJECTIVE To explore the effects of serum Guiluerxian Formula on expression of bone resorption in osteoclasts. METHODS The preosteoclasts of RAW264.7 cell were induced to mature osteoclast by RANKL and M-CSF. Then cultured the mature osteoclast and the Guiluerxian Formula and normal saline （NS） contained serum were respectively added. The expression of TRAP CTsK and CTR mRNA was measured by real time QPCR. RESULTS The Expression of TRAP and CtsK in osteoclast of Guiluerxian group was significantly lower than NS group （P 〈0. 01 ） ,and the CTR is higher than NS group（P 〈0. 01 ）. CONCLUSION Guiluerxian Formu- la can effectively inhibit the expression of bone resorption in osteoclasts.
出处
《海峡药学》
2015年第7期221-224,共4页
Strait Pharmaceutical Journal
基金
福建省卫生厅中医药研究项目(wzgs201301)
福建省教育厅重点项目(JA13513)
关键词
龟鹿二仙胶汤
破骨细胞
骨吸收
Guiluerxian Formula
Osteoclast
Bone resorption
