原子荧光光谱法测定牛黄解毒片中的可溶性砷含量

AFS detecting content of soluble arsenic from Niuhuangjiedu Tablet

目的测定牛黄解毒片的总砷含量以及在人工胃液、人工肠液和水中可溶性砷的含量，了解该复方制剂的砷含量情况，为牛黄解毒片在临床上的合理用药和安全性提供实验依据。方法样品经硝酸、高氯酸、硫酸和盐酸消化后，用硫脲抗坏血酸使五价砷还原为三价砷，再与硼氢化钾反应生成砷化氢气体，载入原子荧光光谱仪测定总砷；样品分别浸泡到人工胃液、人工肠液和水中，经不同时段分别取出上清液进行加酸定容处理，载入原子荧光光谱仪测定可溶性砷。结果可溶性砷在人工胃液及人工肠液中比在水中更早达到溶出平衡。在人工胃液中可溶性砷占总砷含量的2．86％，在人工肠液中可溶性砷占总砷含量的3．13％，在水中可溶性砷占总砷含量的0．11％。结论人工胃液中成人每天可溶性砷最大摄入量为31．38μg，人工肠液中为27．10μg，水中则为15．69μg，未超出世界卫生组织的标准，说明牛黄解毒片可安全服用。

Objective To provide some references for clinically, rationally, and safely using Niuhuangjiedu Tablet and to understand the content of arsenic in Niuhuangjiedu Tablet detecting the total content of arsenic in Niuhaungjiedu Tablet and the content of soluble arsenic in simulated gastric juice and simulated intestinal juice. Methods Niuhuangjiedu Tablets was digested and loaded into AFS. The total arsenic was detected. Niuhuangjiedu Tablets was soaked into simulated gastric juice, simulated intestinal juice, and water. The supernatant fluid was collected in different periods and loaded into AFS. The soluble arsenic was detected. Results Soluble arsenic in artificial gastric juice and intestinal juice reached dissolution equilibrium earlier than that in distilled water. It was found that the content of soluble arsenic in simulated gastric juice accounted for 2.86% of total arsenic in Niuhuangjiedu Tablet and in simulated intestinal juice accounted for 3.13%. Conclusions The highest intake of soluble arsenic in simulated gastric juice in adults is 31.38 μg daily, in simulated intestinal juice 27.10 μg, and in water 15.69 μg. It is not beyond the standard of WHO. Therefore, Niuhuangjiedu Tablet can be safely used.