急性马兜铃酸肾病动物模型建立

The establishment of acute aristolochic acid nephropathy animal model

目的 建立马兜铃酸Ⅰ引起的小鼠急性肾病模型.方法 将小鼠分为实验组与对照组,实验组分别一次性注射马兜铃酸Ⅰ 20、50、100 mg/kg小鼠体重,引起小鼠急性肾功能损伤.并于实验第3、7天取血、肾组织标本,检测相关肾功能指标以及组织形态学检查.结果 注射马兜铃酸Ⅰ后,小鼠肌酐、血尿素氮含量升高,肾脏切片染色显示注射马兜铃酸Ⅰ后肾脏组织形态学变化与注射剂量及时间相关.结论 应用大剂量马兜铃酸Ⅰ成功建立急性肾病小鼠模型.

Objective To establish acute kidney disease mouse model caused by aristolochic acid Ⅰ.Methods The mice were divided into experimental groups and control group,experimental groups were respectively given one-offinjection of aristolochic acid Ⅰ 20,50,100 mg/kg body weight in mice,which leaded to acute renal injury in mice.Took blood and kidney tissue samples on the 3rd,7th day of the experiment,tested related renal function indexes and tissue morphology.Results After the injection of aristolochic acid Ⅰ,the contents of creatinine,blood urea nitrogen of mice increased,the kidney slice staining showed kidney tissue morphological changes were associated with dose and time.Conclusion Application of large dose of aristolochic acid Ⅰ successfully established a mouse model of acute kidney disease.