尼古丁戒断对CCI大鼠痛觉过敏的影响

The effect of nicotine withdrawal on hyperalgesia in CCI rats

目的 探讨尼古丁戒断慢性压迫性损伤（chronic constriction injury,CCI）大鼠痛觉过敏的行为学变化.方法 成年雄性SD大鼠96只,随机分为正常对照组（n=6）、尼古丁戒断组（NT组）、CCI组和NT＋CCI组.NT组、CCI组和NT＋CCI组大鼠按术后1、3、5、7、14 d分为5个亚组,每个亚组6只.观察各组大鼠上述时间点机械刺激缩足阈值（mechanical withdrawal threshold,MWT）、热刺激缩足潜伏期（thermal withdrawal latency,TWL）的变化.结果 与Control组相比,CCI组大鼠术后1、3、5、7、14d MWT显著降低、TWL明显缩短（P＜0.05）,至第7d变化最明显（P＜0.01）;与CCI组比较,NT＋CCI组大鼠术后1、3、5、7、14 d MWT和TWL均降低（P＜0.05）,以术后7d降低最为显著（P＜0.01）.结论 尼古丁戒断引起CCI大鼠痛觉敏感性增高.

Objective To explore the effect of nicotine withdrawal on postoperative hyperalgesia in chronic constriction injury （CCI） rats.Methods 96 adult male SD rats were randomly divided into control group （n=6）,nicotine withdrawal group （NT group）,CCI group and NT＋CCI group.The rats in NT group,CCI group and NT＋CCI group were divided into 5 subgroups according to postoperative 1 d,3 d,5 d,7 d and 14 d,6 rats in each subgroup.The changes of mechanical withdrawal threshold （MWT） and thermal withdrawal latency （TWL） were observed at time points above in each group for every rat.Results Compared with control group,MWT and TWL significantly decreased in CCI group at postoperative 1 d,3 d,5 d,7 d and 14 d （P＜0.05）,to the lowest at the 7th day （P＜0.01）.Compared with CCI group,MWT and TWL both decreased in NT＋CCI group at postoperative 1 d,3 d,5 d,7 d and 14 d （P＜0.05）,to the lowest at the 7th day （P＜0.01）.Conclusion Nicotine withdrawal may raise postoperative hyperalgesia in CCI rats.