防治草莓灰霉病杀菌剂的筛选和复配作用研究

Screening of Fungicides against Strawberry Botrytis cinerea and Study on Synergistic Effect

[目的]筛选对草莓灰霉病菌防治效果较好的复配配方,为防治草莓灰霉病复配制剂的研究奠定基础。[方法]采用室内生长速率抑制法测定腐霉利、烯唑醇、三唑酮、嘧菌酯4种杀菌剂对草莓灰霉病菌菌丝生长的毒力,并研究腐霉利和烯唑醇的复配效果。[结果]4种药剂对草莓灰霉菌均有较强的抑菌活性,其中腐霉利和烯唑醇的抑菌效果最好,EC50分别为0.521 2和0.752 8μg/ml。腐霉利和烯唑醇不同比例的复配对草莓灰霉菌的抑菌活性均表现出相加作用,配比为4∶1时相加作用最明显,共毒系数(CTC)为111.737 4。[结论]腐霉利和烯唑醇按4∶1的比例复配具有杀菌谱广、降低用量、延缓抗药性等优点,可望进一步研究开发。

[Objective] The complex formulation of fungicides against strawberry Botrytis cinerea was screened out to lay the basis for re-preparation study on strawberry Botrytis cinerea. [Method] Antibiotic activities of fungicides like Procymidone,Diniconazole,Triadimefon and Azoxystrobin against strawberry Botrytis cinerea Pers. were studied by bioassay,and synergistic effect of Procymidone and Diniconazole was studied.[Result]The 4 fungicides had exhibited antibiotic activity against Botrytis cinere and the activities of Procymidone and Diniconazole were obvious,their values of EC50 were 0. 521 2 and 0. 752 8 μg / ml,respectively. All blends of Procymidone and Diniconazole with different mass ratio had addition effect against Botrytis cinerea Pers,and when the mass ratio of Procymidone and Diniconazole was 4∶ 1,the effect was the most obvious addition effect,and the value of co-toxicity coefficient was 111. 737 4. [Conclusion] When the mass ratio of Procymidone and Diniconazole was 4∶ 1,the blends had good control spectrum,low dosage and decreasing resistance,so it could be explored further.