鸡卵清蛋白食物过敏大鼠中药口服治疗的实验研究

Oral Administration of Chinese Herbal Formula for OVA-Induced Food Allergy in Rats

目的建立鸡卵清蛋白(OVA)食物过敏大鼠模型,利用中药复方Formula-3对食物过敏大鼠进行治疗,探讨其治疗效果及其机制。方法将24只BN大鼠按随机数字表法分为PBS对照组、OVA模型组及中药复方Formula-3治疗组,每组8只。OVA模型组与中药复方Formula-3治疗组在前6周和第7、9、11、13周分别予OVA(1mg·mL-1)灌胃致敏,每天1次;于第13周末以OVA(100mg·mL-1)进行激发,建立大鼠OVA食物过敏模型。其中中药复方Formula-3治疗组于第7周开始同时给予1mL中药复方Formula-3(150mg·mL-1)灌胃治疗,每天1次;OVA模型组则以1 mL的0.1 mmol·L-1 PBS灌胃治疗。PBS对照组致敏、激发和治疗均予以浓度为0.1mmol·L-1的PBS 1mL灌胃。激发24h后处死大鼠,测定3组大鼠血清中OVA特异性IgE水平、计算肥大细胞脱颗粒的百分比、观察肠道组织超微结构病理变化等,对治疗效果进行评价。结果OVA模型组血清中OVA特异性IgE抗体显著高于PBS对照组、脱颗粒肥大细胞数量显著多于PBS对照组(均P<0.01);中药复方Formula-3治疗组血清中OVA特异性IgE水平显著低于OVA模型组、肥大细胞脱颗粒显著少于OVA模型组(均P<0.01)。OVA模型组肠黏膜微绒毛变形,细胞内细胞器损伤,细胞间连接破坏,炎性细胞浸润增多;中药复方Formula-3治疗组微绒毛均匀整齐,细胞器基本正常,细胞间连接紧密,与OVA模型组相比病变明显轻微。结论中药复方Formula-3能有效地降低食物过敏大鼠血清中OVA特异性IgE,减少肥大细胞脱颗粒并减轻肠道的病理改变,对食物过敏有良好的治疗效果。

Objective To establish a rat model of ovalbumin （OVA）-induced food allergy,and to investigate the therapeutic effect of Chinese herbal Formula-3 on food allergy and its mecha-nism of action.Methods Twenty-four Brown-Norway rats were randomly divided into three groups,with 8 rats in each group.Rats in OVA model group and Chinese herbal Formula-3 treat-ment group were sensitized by OVA gavage 1 mg·mL-1 once daily for 6 consecutive weeks and at weeks 7,9,11 and 13 and were challenged with OVA 100 mg·mL-1 at the end of week 13 to establish a rat model of OVA-induced food allergy.In addition,rats in Chinese herbal Formula-3 treatment group and OVA model group were intragastrically given Chinese herbal Formula-3 150 mg·mL-1 and PBS 0.1 mmol·L-1 once daily from the 7th week,respectively.Rats in PBS＆nbsp;control group only received PBS 0.1 mmol· L-1 during the experimental period.All rats were sacrificed 24 hours after challenge and serum levels of OVA-specific IgE were measured.Further-more,the percentage of mast cell degranulation was calculated,pathological changes in intestinal tissue ultrastructure were observed,and therapeutic effect was evaluated.Results Compared with OVA model group,serum level of OVA-specific IgE and number of degranulated mast cells de-creased in PBS control group,but increased in Chinese herbal Formula-3 treatment group （P 〈0.01）.Rats in OVA model group showed intestinal microvillus deformation,organelle injury,cell junction damage and inflammatory cell infiltration.However,all of these pathological changes in-duced by OVA were ameliorated obviously by treatment with Chinese herbal Formula-3.The rats in Chinese herbal Formula-3 treatment group had uniform microvilli,normal organelles and tight cell junctions.Conclusion Chinese herbal Formula-3 can reduce serum OVA-specific IgE levels, inhibit mast cell degranulation and relieve intestinal pathological changes.Therefore,Chinese herbal Formula-3 is effective for food allergy.