发酵车间空气源细菌淀粉酶产生菌筛选及多样性分析

Screening & Biodiversity of Air Borne Amylase-Producing Strains from a Liquor Fermentation Workshop

采用平板水解圈法(plate hydrolysis spot method)对分离自酒鬼酒发酵车间空气样品的细菌进行淀粉酶产生菌筛选,运用基于16S rRNA基因序列的分析方法对高酶活菌株进行系统发育多样性分析。结果表明,73个受试菌株中,有23株为淀粉酶产生菌,占受试菌株的31.5%,其中有9株为高酶活菌。23个淀粉酶产生菌类群多样性和物种多样性较高,属于4个大的系统发育类群(Actinobacteria、Deinococcus-Thermus、Firmicutes、Proteobacteria)中的10个科(Bacillaceae、Deinococcaceae、Intrasporangiaceae、Microbacteriaceae、Micrococcaceae、Nocardiaceae、Propionibacteriaceae、Pseudomonadaceae、Rhodobacteraceae、Xanthomonadaceae)的13个属,可分为21个物种。进一步分析表明,9株高酶活菌属于细菌域(Eubacteria)的4个大的系统发育类群(Actinobacteria、Deinococcus-Thermus、Firmicutes、Proteobacteria)的7个科(Bacillaceae、Deinococcaceae、Micrococcaceae、Microbacteriaceae、Nocardiaceae、Rhodobacteraceae、Xanthomonadaceae),归属于8个属。研究结果表明,酒鬼酒发酵车间空气源细菌存在较高比例的淀粉酶产生菌,且这些菌株具有较高的类群多样性和物种多样性。

Bacteria collected from air in a liquor fermentation workshop in Jiugui Liquor Co. Ltd. , China, were isolated using plate hydrolysis spot method and screened for their amylase activity. The high amylase active strains were analyzed with 16S rRNA for their diversities of phylogeny. The results showed that 23 out of 73 （31.5%） strains tested were positive in amylase production. Among them 9 strains possessed fairly strong amylase activities. The results based on 16S rRNA phylogenetie analyses showed that the 23 amylase positive strains highly had group and species di- versities belonging to 13 genera （Arsenicicoccus, Arthrobacter, Bacillus, Deinococcus, Janibacter, Kocuria, Luteococcus, Microbacterium, Micrococcus, Paracoccus, Pseudomonas, Rhodococcus, Xanthomonas） of 10 families （ Bacillaceae, Deinococcaceae, Intrasporangiaceae, Microbacteriaceae, Micrococcaceae, Nocardiaceae, Propionibacteriaceae, Pseudomonadaceae, Rhodobacteraceae, Xanthomonadaceae） in 4 phylogenetic groups （ Actinobacteria, Deinococcus-Thermus, Firmicutes, Proteobacteria） and subdivide into 21 species. Further analyses suggested that the 9 strong amylase active strains belonged to 8 genera of 7 families （Bacillaceae, Deinococcaceae, Microbacteriaceae, Micrococcaceae, Nocardiaceae, Rhodobacteraceae, Xanthomonadaceae） in 4 phylogenetic groups （ Actinobacteria, Deinococcus-Thermus, Firmieutes, Proteobaeteria） ： JSM 2215002 and JSM 2215134 belonged to the genus Paracoccus, JSM 2215012 Micrococcus, JSM 2215017 Arthrobacter, JSM 2215018 Microbacterium, JSM 2215076 Bacillus, JSM 2215104 Xanthomonas, JSM 2215109 Deinococcus, and JSM 2215126 Rhodococcus. The results showed that liquor fermentation workshop in Jiugui Liquor Co. Ltd. existed fairly high proportion of amylase producing strains and they highly had groups and species diversities.