高效液相色谱法同时测定饮料中的7种人工合成色素

Simultaneous Determination of Seven Synthetic Colorants in Beverage by HPLC

样品经自制聚酰胺粉固相萃取柱富集和净化后进行HPLC分析。采用Agilent Eclipse XDB-C18（4.6mm×150mm×4.6μm）色谱柱,以甲醇/乙腈（1∶2,体积比）和乙酸铵（0.02mol/L）为流动相进行梯度洗脱,流速1.0mL/min,检测波长为254nm和627nm,分析时间15min左右。7种人工合成色素在1.00～20.0mg/L质量浓度范围内线性关系良好（r均大于0.9999）,加标回收率在89.0%～101%之间,相对标准偏差0.4%～3.4%。该方法操作简便,结果准确可靠,重复性好,适用于饮料中多种人工合成色素的同时检测。

Purified with SPE and concentrated,the sample was separated on an Agilent Eclipse XDB-C18 column with methanol/acetonitrile（1∶2,V/V） and 0.02 mol/L ammonium acetate mixture solution as the mobile phase under gradient elution.The flow rate was 1.0 m L/min.The detection wavelengths were 254 nm and 627 nm.Seven synthetic colorants exhibited excellent linearity over a range of 1.00 ～ 20.0 mg/L（r〉 0.9999）.The mean recoveries ranged from89.0% to 101%.The relative standard derivation was from 0.4% ～ 3.4%.The proposed method is simple,reliable and reproducible,and especially suitable for the detection of synthetic colorants in beverage.