基于硅氧烷喷墨打印加工纸芯片的研究

Fabrication of a Microfluidic Paper-Based Analytical Devices by Ink Jet Printing of Siloxane

提出了一种基于硅氧烷喷墨打印技术加工纸芯片的方法.将十八烷基三甲氧基硅烷的正庚烷溶液以喷墨打印的方式打印到滤纸上,由于滤纸的强吸水性,十八烷基三甲氧基硅烷的甲氧基水解生成硅羟基,硅羟基与滤纸纤维上的羟基缩合使硅氧烷结合到滤纸纤维上.该加工方法简单、低廉,适于纸芯片的大规模加工与制作.用该方法加工的纸芯片分析了尿样中的蛋白质含量,证明其在临床检测中的广泛应用前景.

In this paper, a method for fabrication of microfluidic paper-based analytical device is developed by printing of siloxane.Trimethoxyoctadecylsilane（TMOS）-heptane solution was printed onto the filter paper using an inkjet printer, the TMOS was hydrolyzed to produce Si-OH owing to the water absorbed in the filter paper, allowing the immobilization of TMOS onto the cellulose via the reaction between Si-OH and cellulose OH.Thus, the printed area was patterned hydrophobic while other area maintained hydrophilic.This method is simple and cost-effective, allowing the mass production of microfluidic paper-based Analytical devices.The fabricated device was used to determine the protein in urine samples, demonstrating its great potential in clinical diagnostics.