摘要
目的:探讨miR-125b在人胶质瘤组织中的表达。方法:选取不同恶性程度胶质瘤手术患者20例为胶质瘤组(Ⅰ级5例、Ⅱ级5例、Ⅲ级5例、Ⅳ级5例),另选择实施脑外伤内减压手术的患者5例为正常对照组。术中取下胶质瘤组胶质瘤组织和正常对照组的正常脑组织,采用荧光定量PCR方法检测miR-125b的表达。结果:正常对照组的miR-125b相对表达量为(0.28±0.12),胶质瘤组Ⅰ、Ⅱ、Ⅲ、Ⅳ级的miR-125b相对表达量分别为(0.43±0.07)、(0.69±0.08)、(0.90±0.05)、(1.12±0.04),差异有统计学意义(P<0.05)。肿瘤病理恶性程度越高,生存期越短(P<0.05)。结论:miR-125b异常表达可能与胶质瘤的发生和发展密切相关。
Objective：To explore the expression of miR-125b in human glioma samples. Methods： Twenty patients with different malignant degrees of gliomas were selected as gliomas group （5 cases in grades I - Ⅳ）. Five patients with cerebral trauma decompression surgery were enrolled as normal controls. The expression of miR-125b in glioma samples and normal brain samples was detected by fluorescence quantitative PCR. Results： The expression levels ofmiR-125b in the normal control group was （0.2_8±0.12）, and the expression levels ofmiR-125b in different grades （I,II,III^IV） of gliomas group were （0.43 ±0.07）, （0.69±0.08）, （0.90±0.05）, （1.12±0.04） respectively. The expression levels ofmiR-125b were statistically different between the 2 groups （P〈0.05）. The pathological grade of the tumor pathology was associated negatively with the survival time in patients （P〈0.05）. Conclusion： The abnor- mal expreesion ofmiR-125b is probably correlated to the progress and development of glioma.
出处
《神经损伤与功能重建》
2015年第4期309-311,共3页
Neural Injury and Functional Reconstruction
