G-CSF对东莨菪碱痴呆模型大鼠认知及ChAT表达的影响

Effect of G-CSF on the expression of Ch AT and cognitive function of dementia model rats induced by scopolamine

目的观察粒细胞集落刺激因子(G-CSF)对东莨菪碱所致大鼠认知障碍和Ch AT表达的影响。方法24只10月龄Wistar雄性大鼠随机分为三组:空白组(N组)、东莨菪碱组(S组)和G-CSF干预组(G组),分别腹腔注射生理盐水2 ml(N组)、氢溴酸东莨菪碱1 mg/kg(S组)、腹腔注射氢溴酸东莨菪碱1 mg/kg后皮下注射GCSF 50μg/kg,连续5 d。每天给药后3 h进行水迷宫测试其空间学习记忆能力改变,末次水迷宫实验后4%高聚甲醛灌注取脑组织用免疫组化方法观察Ch AT阳性细胞表达。结果行为学变化:各组大鼠第5天潜伏期和游泳距离较第1天短(P<0.05),N组和G组大鼠第5天潜伏期和游泳距离均较S组短(P<0.05)。Ch AT表达变化:N组和G组大鼠海马Ch AT阳性细胞个数均较S组多(P<0.05)。结论 G-CSF可以有效改善东莨菪碱所引起的大鼠认知功能障碍。

Objective To observe the effects of G-CSF on scopolamine-induced cognitive impairment and ChAT expression in rats-model. Methods Twenty-four Wistar rats aged 10 months were randomly divided into three groups：blank group （group N）,Scopolamine group （group S） and G-CSF treatment group（group G）. Group N was injected normal saline 1ml intraperitoneally,group S scopolamine hydrobromide 1 mg/kg intraperitoneally,and group G G-CSF 50μg/kg subcutaneously after injection of scopolamine hydrobromide 1 mg/kg intraperitoneally,for continuous 5 days. Three hours after giving due doses the water-labyrinth examination was conducted every day for testing its spacial alteration of learning remember aptitude. After the final examination paraformaldehyde perfused brain was taken to observe ChAT positive cell expression by using immunohistochemical method. Results The changes in behavior：the incubation period and swimming distance of each group by navigation test were shorter on the fifth day than that on the first day （P〈0.05）. Compared with group S,The incubation period and swimming distance on the fifth day of group N and G were significantly reduced （P〈0.05）. The changes in the expression of ChAT：in Group N and G, the quantity of the ChAT positive cell was larger than that in Group G （P〈0.05）. Conclusion Granulocyte colony stimulating factor （i.e G-CSF） can effectively improve the condition of rats with cognitive dysfunction induced by scopolamine.