新型人工生物补片作为主动脉瓣替代材料的可行性

Feasibility of New Artificial Biologic Patch as Aortic Valve Replacement

目的探讨胶原膜与碱性成纤维细胞生长因子(base fibroblast growth factor,b FGF)特异结合的人工生物补片作为主动脉瓣膜替代材料的可行性。方法健康杂种家犬10只随机分为对照组和实验组,每组5只,静脉全麻、腹主动脉阻断下分别使用仅浸泡磷酸盐缓冲液的胶原膜材料或特异结合b FGF的胶原膜材料作为人工瓣膜行腹主动脉腔内移植。于术后3个月利用超声心动图对人工瓣膜的功能进行评价,于术后6个月取材观察人工瓣膜状况。结果术后3个月超声心动图检测人工瓣膜的功能在对照组和实验组之间无差别,术后6个月对照组及实验组犬腹主动脉腔内的人工瓣膜均降解。结论胶原膜与b FGF特异结合的人工生物补片在体内的降解速率较快,目前尚不能作为主动脉瓣膜良好的替代材料。

Objective To study the feasibility of artificial biologic patch made of collagen membranes loaded with collagen-binding base fibroblast growth factor （ bFGF） as aortic valve replacement.Methods Ten healthy mongrel dogs were randomly divided into control group and experiment group （ both n=5 ） .Abdominal aorta replacement using artificial biologic patch made of collagen membranes loaded with collagen-binding bFGF （ experiment group） or soaked with phosphate buffered saline （ control group） was performed under general anes-thesia and abdominal aorta blockage.Three months after the surgery, echocardiography was performed to evaluate the function of the artificial valve.Six months after the surgery, the condition of the artificial valve transplanted in the abdominal aorta was observed.Results No significant difference was found between the experiment group and the control group in function of the artificial valve evaluated by echocardiography 3 months after the surgery. The artificial valves entirely degraded 6 months after implanted into the abdominal aorta in both groups.Conclu-sion The degradation of artificial biologic patch made of collagen membrane loaded with collagen-binding bFGF in vivo is so fast that it is not a good alternative for replacement of the aortic valve.