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ERK1/2通路在Peroxiredoxin-1抑制TGF-β_1诱导的肺成纤维细胞增殖中的作用 被引量：4

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摘要 目的探讨细胞外信号调节激酶1/2(ERK1/2)在新型过氧化物酶Peroxiredoxin-1(Prx-1)抑制转化生长因子-β1(TGF-β1)诱导的肺成纤维细胞增殖中的作用。方法体外培养肺成纤维细胞,随机分为对照组、TGF-β1组、阴性转染组、Prx-1 siRNA转染组。各组细胞用0.4%血清培养12 h使其同步化。TGF-β1组、阴性转染组和Prx-1 siRNA转染组均添加5μg/L TGF-β1刺激24 h,阴性转染组和Prx-1 siRNA转染组分别利用脂质体Lipo2000将干扰siRNA和Prx-1 siRNA转染到肺成纤维细胞,培养48 h。Real-time PCR检测转染后Prx-1 mRNA表达;MTT检测细胞增殖、Western blot检测ERK1/2及Prx-1表达、2,7-二氯荧光素二乙酸(DCFHDA)检测活性氧(ROS)水平。结果 Prx-1 siRNA转染肺成纤维细胞后,Prx-1 mRNA表达明显降低,最大抑制率为92%。与对照组比较,TGF-β1组的细胞增殖、ROS、磷酸化ERK1/2(p-ERK1/2)及Prx-1蛋白的表达水平均明显增加。与TGF-β1组比较,阴性转染组中的上述观察指标无明显变化,但Prx-1 siRNA转染组的细胞增殖、ROS、p-ERK1/2的水平明显上升,而Prx-1蛋白的表达被抑制。结论 TGF-β1能够诱导肺成纤维细胞生成ROS,并由此促进ERK1/2的激活,最终导致细胞增殖,而Prx-1 siRNA则可通过增加ROS水平进一步促进TGF-β1的作用。
作者 孙影 刘宝欣 魏中秋 冯莉 范玉磊 梁婷婷 杨方
机构地区 华北理工大学基础医学院病理学系 唐山工人医院呼吸内科 华北理工大学实验中心
出处 《广东医学》 CAS 北大核心 2015年第14期2139-2141,共3页 Guangdong Medical Journal
基金 国家自然科学基金资助项目(编号:81072254) 2014年河北省唐山市科学技术研究与发展计划(第三批)项目(编号:14130275B)
关键词 Prx-1 活性氧 细胞外信号调节激酶1/2 转化生长因子-Β1 细胞增殖
分类号 R135.2 [医药卫生—劳动卫生]
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参考文献10

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同被引文献47

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广东医学
2015年 第14期

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