具核梭杆菌感染与结直肠癌的关系

Association of Fusobacterium nucleatum infection with colorectal cancer

目的探讨具核梭杆菌(Fn)感染与结直肠癌(CRC)的相关性。方法对手术切除、经病理证实的101例CRC组织和配对正常组织进行实时荧光定量PCR(FQ-PCR)检测组织中Fn丰度。随机抽选其中22例CRC和配对正常组织进行荧光原位杂交,验证FQ-PCR的结果,分析Fn的富集度与临床病理指标的相关性。结果两检测方法均证实Fn在CRC组织中富集,CRC组织中Fn丰度显著高于配对正常组织者占87.1%(88/101),CRC组织Fn丰度归一化值为0.242±0.034,显著高于配对正常组织的0.050±0.017(P=0.000)。Fn的高丰度与患者的性别、年龄、肿瘤位置、分化程度、浸润程度、Dukes分期等临床病理指标均无显著相关性(P>0.05),而与淋巴转移密切相关(P<0.05)。结论 Fn在CRC组织中富集,并有可能促进淋巴结转移。

Objective To investigate whether Fusobaeterium nucleatum （Fn） infection is associated with colorectal cancer （CRC）. Methods The resected CRC （pathologically confirmed） mass and matched normal samples from 101 patients were collected. Quantitative fluorescent polymerase chain reaction （FQ -PCR） was applied to detect Fn in CRC versus matched normal tissues. Fluorescence in situ hybridization （FISH） analysis was performed on 22 randomly selected CRC and matched samples to confirm FQ - PCR results. Abundance of Fn was analyzed for association with clinicopathological parameters. Results Both analyses demonstrated that Fn was significantly over - represented in CRC. The rate of Fn over -abundance in CRC tissues versus that in matched normal tissues was 87.1% （88/101）. The abundance of Fn was significantly greater in tumor tissues （0. 242 ＋ 0. 034） than in matched normal tissues （ 0. 050 ±0. 017） （ P = 0. 000）. No significant association of Fn load with patients＇ gender, age, cancer location, stages and infiltration （P 〉 0. 05 ） but with lymph node metastases （P = 0. 000 ） was observed. Conclusion Fn is enriched in the CRC tissues, which may be associated with CRC development and promote lymph node metastasis.