四川地区猪肠外致病性大肠杆菌的分离鉴定与耐药性检测

Isolation,Identification and Antimicrobial Resistance of Extraintestinal Pathogenic Escherichia Coli From Swine in Sichuan Area

本试验对四川地区52个猪场送检的210个病死猪样本进行了肠外致病性大肠杆菌的分离鉴定和耐药性检测。通过培养特性、染色特点、形态观察、PCR鉴定及致病性试验，共分离到40株肠外致病性大肠杆菌．其中以肺脏和淋巴结的分离率最高。40株肠外致病性大肠杆菌中，有34株同时耐6种以上的受试药物，其中对四环素、氨苄西林和卡那霉素的耐药率最高（大于80％），对喹诺酮类药物的耐药率为50％～52．5％。对氨苄西林，舒巴坦、阿米卡星、头孢克肟保持较高的敏感性，耐药率小于20％。

This paper studied the isolation, identification and amimicrobial resistance of extraintestinal pathogenic Escherichia coli （ExPEC） from 210 pig＇s samples in Sichuan province. Forty ExPEC strains were collected by morphological, cultural character, pathogenicity test and PCR assay. Most of the ExPEC strains were isolated from lungs and lymph nodus. The antimicrobial resistance test showed that 34 ExPEC strains resistant to 6 antimicrobials at least and the resistance to tetracycline, ampicillin and kanamycin was highest （resistance rate 〉80% ）, followed by fluoroquinolones （resistance rate was 50.0%-52.5%）, all ExPEC strains had higher susceptibility to ampicillin-sulbactam, amikacin and eefixime {resistance rate 〈20% ）.