基于重组自交系的水稻抗纹枯病QTL定位（英文）

Mapping of QTLs for Sheath Blight Resistance Using Recombinant Inbred Lines of Rice(Oryza sativa L.)

利用157个家系组成的大关稻/IR28重组自交系群体,采用牙签接种法,鉴定亲本及157个重组自交系群体对水稻纹枯病的抗性。利用QTL Cartographer软件,对水稻纹枯病抗性基因进行检测分析。结果表明,共检测到qsb1、qsb2、qsb5-1和qsb5-2 4个QTL位点,分别位于第1、2和5染色体上,贡献率为10.41%~36.92%。qsb5-1的加性效应为负值,表明来自供体亲本IR28相应QTL使纹枯病病斑比值变小;qsb1、qsb2和qsb5-2的加性效应为正值,表明来自供体亲本大关稻相应QTL使纹枯病病斑比值变大。

A recombinant inbred line （RIL） population composed of 157 lines derived from an inter-subspecific hybrid of Daguandao/IR28 by the single seed descent method was used as materials, and the quantitative trait loci （QTLs） coffering the resistance to sheath blight in the 157 RILs and the parents were detected using the toothpick inoculation method. The disease indexes of rice sheath blight in the two parents and 157 RILs were scored and the QTLs responsible for rice sheath blight resistance were detected accordingly by QTL Cartographer software. The results showed that a total of 4 QTLs （qsbl, qsb2, qsb5-1, qsb5-2） conferring sheath blight resistance were detected on chromosomes 1, 2 and 5, and their variance explained ranged from 10.41% to 36.92%. The additive effect of qsb5-1 was negative, indicat- ing that the QTLs derived from donor parent IR 28 could enhance the resistance to sheath blight. However, the additive effects of qsbl, qsb2 and qsb5-2 were positive, indicating that the QTLs derived from donor parent Daguandao weakened the resis- tance to sheath blight.