淹水胁迫对黄山栾树幼苗渗透物质与荧光特性的影响

The Effect of Waterlogging Stress on Osmolytes and Fluorescence Characteristics of Koelreuteria bipinnata Franch. var. integrifoliol Seedlings

淹水胁迫试验采用双盆套法对黄山栾树幼苗分别进行正常浇水(CK)、涝害处理(ZK)及渍害处理(LK),同时观察在3种处理方式下其生长状况、渗透调节物质含量及荧光特性的变化,研究发现第31天ZK幼苗叶片几乎全部脱落,而LK幼苗叶片则部分变黄。随着淹水时间的延长,ZK幼苗、LK幼苗的脯氨酸含量、可溶性糖含量、Fo值、NPQ值均大幅度提高,分别提高了301%与263%、220%与144%、20.61%与8.2%、86.84%与38.99%。而Fm、Fv/Fm、ETR大幅度下降,分别降低了78.89%与42.80%、73.63%与45.25%、77.71%与53.50%,但变化幅度LK幼苗比ZK幼苗小很多,这一结果与其外观形态特征变化较为一致。可见,涝害比渍害对黄山栾树幼苗生长影响明显大得多,较长时间渍害下黄山栾树可能会存活,但较长时间涝害下可能会死亡。

The way of double pots was taken in this flooding stress experiment, and three treatments were set which were normal watering（CK）, water logging（ZK） and subsurface watering（LK） to research the influence on growth, osmotic substance content and fluorescence properties of the Koelreuteria bipinnata Franch. var. integrifoliola seedlings. The result showed that the Leaves almost fell at the 31 d under the treatment of ZK, and part of the leaves under treatment of LK turned yellow. With the extension of waterlogging time, the proline contents, soluble sugar contents, Fo value and NPQ value of ZK seedlings and LK seedlings are increase significantly. And it respectively increases by 301% and 263%, 220% and 144%, 20.61% and 8.2%, 86.84% and 8.2%. While the Fm, Fv/Fm, ETR of them drop substantially, reduce by 78.89 and 42.80%, 73.63% and 45.25%, 77.71% and 53.50%. But the rangeability in LK seedlings is much smaller than in ZK seedlings. This result is consistent with the appearance of morphological changes. It shows that the effects of ZK is much larger than LK for Koelreuteria bipinnata Franch. var. integrifoliola Seedlings. A long time of waterlogging, Koelreuteria bipinnata Franch. var. integrifoliola may survive, but a long time of waterlogging, it may die.