三个牦牛品种(类群)尿苷一磷酸合成酶缺乏症的检测

Detection of deficiency of uridine monophosphate synthase in three yak breeds or population

检测牦牛中是否存在尿苷一磷酸合成酶缺乏症(DUMPS).实验选取分布于四川省的昌台牦牛、九龙牦牛、麦洼牦牛三个品种(类群)共100头,从血液中提取基因组DNA,采用常规PCR-RFLP技术进行DUMPS的遗传缺陷检测.首先扩增牦牛尿苷一磷酸合成酶基因部分片段,再用限制性内切酶AvaⅠ酶切扩增产物,通过酶切条带数确定牦牛群体中是否存在DUMPS携带者.结果显示,所检测的100头牦牛PCR产物的酶切条带均为3条,为正常纯合子,未发现DUMPS携带者.

The aim of the study is to detect the occurence of yaks carrying deficiency of uridine monophosphate synthase （DUMPS）. A total of 100 yaks belonging to three breeds or population in Sichuan Province（ Changtai yak ,Jiulong yak and Mai- wa yak）were used, Genomic DNA was extracted from the blood, and PCR-RFLP was employed to screen the possible occurrence of DUMPS. Ava I was used to digest PCR product generated by amplification of genomic DNA using DUMPS specific primers, and the genotypes were visualized according to the sizes of the fragments. The results showed that no carrier of DUMPS was found among the 100 yaks examined, normal homozygous DUMPS allele exhibited three fragmehts.