细辛炮制前后的药效学及毒理学研究

Pharmacodynamics and toxicology studies of Asarum before and after processing

目的研究细辛炮制前后抗炎镇痛的药效学及其对小鼠的毒性作用。方法采用醋酸扭体实验、热板实验评价细辛的镇痛作用,采用二甲苯致小鼠耳廓肿胀实验评价细辛的抗炎作用,采用小鼠急性毒性实验观察细辛炮制前后对小鼠急性毒性半数致死量(LD_50)的作用。结果与生理盐水组比较,其他5组小鼠疼痛潜伏期均延长,扭体次数均减少,足肿胀度及耳廓肿胀度均降低,差异有统计学意义(P<0.05或P<0.01)。细辛生品水煎液高剂量组扭体次数[(11.01±2.83)次]多于细辛炮制品水煎液高剂量组[(8.71±2.04)次](P<0.05)。细辛生品水煎液高剂量组给药后1、2 h足肿胀度[(13.00±1.86)、(14.00±2.30)mg]高于细辛炮制品水煎液高剂量组[(9.00±1.99)、(10.00±2.30)mg],差异有统计学意义(P<0.05)。细辛生品水煎液低剂量组耳廓肿胀度[(13.30±1.35)mg]高于细辛炮制品水煎液低剂量组[(9.70±1.41)mg],差异有统计学意义(P<0.05)。细辛生品水煎液低剂量组对二甲苯诱导的小鼠耳廓炎症抑制率(17.30%)显著低于细辛炮制品水煎液低剂量组(22.80%)(P<0.05)。结论细辛炮制后急性毒性显著降低,并且炮制后抗炎镇痛作用强于生品。

Objective To research anti-inflammatory analgesic pharmacodynamics and toxic effects of Asarum on mice before and after processing. Methods Body torsion test by acetic acid and hot plate test were used to evaluate the analgesic action of Asarum. Xylene induced mouse ear swelling test was used to evaluate the anti-inflammatory effects of Asarum. Acute toxicity in mice was used to observe the effect on acute toxicity in mice half lethal dose（LD50） before and after the Asarum processing method. Results Compared with saline group, pain latency in other 5 groups of mice were prolonged, the times of twisting body in other 5 groups of mice were reduced, foot swelling degree and auricle swelling degree in other 5 groups of mice were reduced, with statistical difference（P〈0.05 or P〈0.01）. The times of twisting body in crude Asarum decoction high dose group [（11.01±2.83） times] was more than that in processed product Asarum decoction high dose group [（8.71±2.04） times]（P〈0.05）. Foot swelling degree after administration of 1,2 h in crude Asarum decoction high dose group [（13.00±1.86）,（14.00±2.30） mg] were higher than those in processed product Asarum decoction high dose group [（9.00 ±1.99）,（10.00 ±2.30） mg], with statistical difference（P〈0.05）. Ear swelling degree in crude Asarum decoction low dose group [（13.30±1.35） mg] was higher than that in processed product Asarum decoction low dose group [（9.70±1.41） mg], with statistical difference（P〈0.05）. Xylene induced mouse ear inflammation inhibition rate in crude Asarum decoction low dose group（17.30%） was significantly lower than that in processed product Asarum decoction low dose group（22.80%）, with statistical difference（P〈0.05）. Conclusion Asarum acute toxicity decreased significantly after processing, and anti-inflammatory analgesic action of processed products is stronger than that of raw products after processing.