大鼠子宫全层损伤修复模型的建立

Establishing of rat uterus repair model after full-thickness injury

目的：建立SD大鼠子宫全层损伤后修复模型,并探索子宫损伤后形态及功能重建的规律。方法：SD大鼠子宫全层损伤,根据损伤范围不同分为A组（0.5cm×0.5 cm）、B组（1.0cm×0.5cm）、C组（1.5cm×0.5cm）、D组（假手术）四组。术后1周、2周、4周、8周、12周,对子宫创面的愈合情况行大体观察及组织学分析。术后12周行妊娠试验。结果：大体观察显示, A、B、C 组的子宫术后宫腔通畅率分别为96%、84%和56%。术后1周,3组手术区即可见上皮再生。术后2~4周,Masson染色及免疫组化染色可见平滑肌修复,同一时间点A、B、C 3组手术区平滑肌细胞含量（α-SMA）比较,差异有统计学意义。术后12周,4组的妊娠率比较差异有统计学意义（P=0.006）,A组手术区可见正常发育至晚孕期的胚胎,B组、C组手术区胚胎少见或未见。结论：全层机械损伤大鼠子宫模型是理想的子宫损伤修复动物模型。子宫损伤后形态学、功能学的自然修复与损伤程度有关,损伤范围增大,子宫自然修复不能实现完全重建,有待进一步干预。

Objective：To evaluate the histological and functional repair process of rat uterus after full-thickness injury. Methods：The method uses as a model the SD rat uterine horn. According to the different degree of injury,70 SD rats were divided into four groups：Group A（0. 5cm×0. 5cm）,Group B（1. 0cm×0. 5cm）,Group C（1. 5cm×0. 5cm） and Group D （sham surgery）. After l,2,4,8,12 weeks,the animals were sacrificed and the uterine horns were prepared for gross and histologic evaluation. After 12 weeks,fecundability was tested. Re-sults：The horns of Group A showed 96% patency and had developed morphology similar to nor-mal uterus. The patency rates of Group B and C were 84% and 56%. After 1 week,we observed re-epithelialization of the injured tissue. After 2~4 weeks,a few smooth muscle cells were ob-served with Masson stain and immunohistochernical staining. There was significant statistical difference between Group A and Group B or C. Uterine horn of female rat mated 12 weeks after surgery. In Group B and C,most embryos were implanted in normal areas instead of scar sites. Conclusions：The modelling of uterine wound repair model was performed successfully by using SD rat. The wound healing of the uterine horns is affected by the different degree of full-thick-ness injury. For Group B and C,measures such as tissue engineering may be needed for better repair.