摘要
试验研究了影响玫瑰冠性状、鱼腥味性状和矮小性状的MNR2、FMO3和GHR基因突变位点在林甸鸡群中的分布情况。通过PCR对分别影响林甸鸡玫瑰冠性状、鱼腥味性状和矮小性状的MNR2和GHR基因突变位点进行分型,发现林甸鸡群玫瑰冠个体为MNR2-RS型;林甸鸡群中存在鱼腥味易感基因FMO3基因c.984A>T变异位点,25.4%鸡只携带T等位基因,其中TT基因型频率为4.3%,AT基因型频率42.2%;没有检测到影响矮小性状GHR基因第10外显子和3′UTR区1.7 kb缺失(c.1744_3516 del)突变位点。通过追溯试验群体的系谱结构,发现影响林甸鸡玫瑰冠型的MNR2基因、鱼腥味性状的FMO3基因和矮小性状的GHR基因的突变位点在上下代传递过程中与先前报道结果一致。
The rose-comb,fishy taint and dwarf traits are genetically controlled by three different genes,homeodomain protein（MNR2）,flavin containing monooxygenase 3（FMO3）and growth hormone receptor（GHR）,respectively. The aim of this study was to evaluate the allele frequency distribution of these three genes in Lindian chicken population,respectively. The genotypes of MNR2,FMO3 and GHR genes were checked directly by PCR,which showed that all birds with the rose-comb phenotype were of the RS genotype for MNR2 gene. The FMO3 genotype was detected by PCR and electrophoresis. The results showed that 25.4% of Lindian chickens had the c.984A〉T mutation for FMO3 gene,where genotypes TT and AT accounted for 4.3% and 42.2%,respectively. And the 1.7 kb deletion mutations（c.1744_3516 del）was not detected in 10 th exon and 3′UTR of GHR gene affected dwarf traits. By tracing the pedigree relationship of all experimental birds,it was proved that the alleles of MNR2,FMO3 and GHR genes were transmitted consistently with previously reported results.
出处
《中国家禽》
北大核心
2015年第14期7-12,共6页
China Poultry
基金
现代农业产业技术体系建设专项资金(CARS-42)
黑龙江省"两大平原"改革试验农业重点项目(2014)
黑龙江省畜禽良种化工程项目(2011-2012)
黑龙江省高等学校科技创新团队建设项目(2010td02)
