施钼对冬小麦钼高、低效品种氮代谢的影响

Effects of Mo on nitrogen metabolism and the differential analyses of Mo-efficient and Mo-inefficient winter wheat cultivars

采用改良霍格兰营养液试验,研究施钼处理对冬小麦钼高、低效品种氮代谢的影响,并对冬小麦钼高、低效品种对钼的响应差异进行分析。结果表明,施钼处理后冬小麦地上部和地下部硝酸还原酶和谷氨酰胺合成酶的活性提高,硝态氮的含量显著降低,代谢产物铵态氮含量、游离氨基酸总量、可溶性蛋白含量增加。施钼处理对冬小麦氮代谢具有促进作用。施钼处理对冬小麦钼高、低效品种氮代谢影响差异的原因在于2个冬小麦品种的基因型不同。与冬小麦钼高效品种相比,冬小麦钼低效品种施钼处理后硝酸还原酶和谷氨酰胺合成酶的活性以及地上部硝态氮、游离氨基酸、蛋白质、地下部游离氨基酸含量的变化幅度更大。

A modified Hoagland solution culture experiment was conducted to study the effects of Mo on nitrogen metabolism and the difference of Mo-efficient and Mo-inefficient winter wheat cultivars.The results showed that application of Mo significantly increased the activities of nitrate reductase and glutamine synthetase,decreased the content of nitrate.The contents of ammonium,total free amino acids and soluble protein were improved,indicating that the nitrogen metabolism of winter wheat was promoted by applying Mo.Genotypic differences in response of molybdenum on nitrogen metabolism were found between Mo-efficient and Mo-inefficient winter wheat cultivars.The changing rates of nitrate reductase and glutamine synthetase,the contents of nitrate,free amino acids and soluble protein in shoot and free amino acids in root of Mo-inefficient cv.97014 under Mo application were higher than those of Mo-efficient cv.97003.