水产品中杆菌肽残留的微生物法检测

Microbiological inhibition method for determination of bacitracin residues in muscles of aquatic products

以藤黄微球菌（Micrococcus luteus）为检测菌种,样品经甲醇-0.1%甲酸水（体积比为3/7）超声提取,旋蒸浓缩,用杯碟法建立水产品可食性组织（草鱼、鳗鲡和对虾肌肉）中杆菌肽残留的测定方法。结果显示：杆菌肽在草鱼肌肉中最低检测限为0.25μg/g,在对虾和鳗鲡肌肉中最低检测限为0.50μg/g,均达到了我国农业部和欧盟规定的杆菌肽在肌肉组织中的最高残留限量检测要求。杆菌肽在各水产品肌肉组织中1~10μg/mL范围内,药物质量浓度对数与抑菌圈直径的线性关系良好（R2〉0.990 0）,杆菌肽不同水产品肌肉组织添加质量浓度在0.5~2.5μg/g时,各组织的回收率均在70.45%~93.44%范围内,变异系数在2.02%~12.62%。并采用3种不同细菌制作的检定平板确定出残留物为杆菌肽,方法有效。

The cylinder plate method for determination of bacitracin residues in aquatic products（grass carp,eel and prawn muscle）was established.The samples were extracted with methanol and0.1%formic acid water（volume ratio 3∶7）under ultrasonication,reduced pressure distillation and then using Micrococcus luteus to detect residual after the concentration of samples.The results showed that the detection limit of bacitracin was 0.25μg/g in grass carp muscle and 0.50μg/g in eel and prawn muscle,which satisfied the maximum residue standards of muscle tissue ruled by Chinese Ministry of Agriculture,as well as the European Union.When bacitracin in the muscle tissue of aquatic products ranges from 1to 10μg/mL,it showed a good linearity between drug concentration logarithm and the diameter of bacteriostatic（R2〉0.990 0）.For different aquatic products,when additive amount of bacitracin ranged from 0.5μg/g to 2.5μg/g,the recoveries of all muscle tissues were 70.45%-93.44%,and the relative standard deviations were 2.02%-12.62%.Furthermore,three different bacterial test plates was adapted to ensure the residues in aquatic products is bacirtracin.