摘要
目的探讨血清Dickkopf-1（DKK-1）蛋白联合CA199检测在胃癌诊断中的应用价值。方法选取2013年5月-2014年12月本院收治的58例原发性胃癌患者作为胃癌组,同期收治的60例慢性浅表性胃炎患者作为慢性胃炎组,同时收集60例体检健康人作为健康对照组。3组同时应用酶联免疫吸附法测定DKK-1,应用化学发光法测定CA199,并比较DKK-1、CA199诊断胃癌的特异度、敏感度、准确度、阳性似然比、阴性似然比。结果与慢性胃炎组和健康对照组相比,胃癌组血清DKK-1、CA199水平显著升高（P〈0.01）;与单独应用DKK-1或CA199检测相比,DKK-1联合CA199检测的敏感度、准确度、阳性似然比明显升高（P〈0.05）,阴性似然比明显下降（P〈0.05）。结论血清DKK-1可以作为胃癌临床辅助诊断的依据之一,血清DKK-1联合CA199检测对提高胃癌的诊断水平有独特的优势,值得临床推广应用。
Objective To explore the application value of serum Dickkopf-1 （DKK-1） protein combined with CA199 test in the diagnosis of gastric cancer. Methods 58 patients with primary gastric cancer admitted into our hospital from May 2013 to December 2014 were selected as gastric cancer group,60 patients with chronic superficial gastritis in the same time were selected as chronic gastritis group,and 60 eases of medical healthy people were selected as healthy control group.In the 3 groups,ELISA was applied to test DKK-land chemiluminescence was applied to test CA199.The specificity,sensitivity,accuracy,positive likelihood ratio and negative likelihood ratio of DKK-1 and CA199 was compared. Results Compared with chronic gastritis group and healthy control group,levels of serum DKK-1 and CA199 in p gastric cancer group significantly improved （P〈0.01）.Compared with the single application of DKK-1 or CA199,the sensitivity,aeeuracy and positive likelihood ratio of DKK-1 combined with CA199 significantly increased （P〈0.05）,and the negative likelihood ratio significantly decreased （P〈0.05）. Conclusion Serum DKK-1 can be used as a basis for clinical diagnosis of gastric cancer.Serum DKK-1 combined with CA199 test detection has a unique advantage in the diagnosis of gastric cancer,it is worthy of clinic application.
出处
《中国当代医药》
2015年第21期121-123,共3页
China Modern Medicine
