高通量筛选之痛——泛活性筛选干扰化合物

Pain of high-throughput screening——pan assay interference compounds

高通量筛选技术在新药发现中已经成为常规的方法,该技术可以有效地筛选出作用于特定靶点的化合物,同时,这一方法也引入了一些干扰筛选的特殊化合物分子。泛活性筛选干扰化合物以干扰生化实验检测、对蛋白进行共价修饰、自身形成聚集体等方式干扰药物筛选,故清除此类化合物是重要的研究内容。本文对泛活性筛选干扰化合物的概念和分类进行了介绍,并对其产生原因以及相关对策进行综述,以期提高高通量药物筛选的技术水平和命中率。

High-throughput screening is a regular approach available for identifying new lead compounds for the growing validated drug targets in drug screening. However, it has also introduced a large number of peculiar molecules which interfere drug screening. Pan assay interference compounds （PAINS） interfere with the progress of drug screening in various ways, such as interfering with a biochemical assay, modifying the protein, aggregate-based inhibitors and so on. So it is of vital significance to remove them. This paper has consulted the concept, category of PAINS and reviewed the way of PAINS interfering and the countermeasures to cope with them to direct the approach of high through screening and improve the hits percent.