超微七味白术散对肠道厌氧微生物代谢多样性的调控作用

Effects of ultra-micro powder qiweibaizhusan on metabolism diversity of intestinal anaero-microbiotia in diarrheal mice with dysbacteriosis

探讨超微七味白术散对菌群失调腹泻的肠道微生态调节作用。采用同时灌胃头孢拉定和硫酸庆大霉素进行小鼠菌群失调造模,灌胃给药七味白术散传统汤药和超微七味白术散1/2剂量的汤药进行治疗,正常组和模型组灌胃等量的无菌水,治疗3 d后,提取肠道微生物,在厌氧条件下,运用Biolog微生物自动分析系统研究肠道微生物的代谢情况。培养72 h时,各组的颜色平均变化率(AWCD)趋于稳定,且1/2剂量的超微汤药治疗组>正常组>传统汤药治疗组>模型组;正常组的Shannon指数和Simpson指数为最大,且与其他各组差异极显著(P<0.01),1/2剂量超微汤药治疗组的Shannon均匀度和Mclntosh指数最大,Shannon均匀度与正常组差异不显著(P>0.05),其余指标与各组都存在显著性差异(P<0.01或P<0.05)。聚类分析和主成分分析表明抗生素的抑菌作用明显,使小鼠肠道微生物的代谢受到影响,七味白术散传统汤药和1/2剂量超微汤药的调控使小鼠肠道微生物的代谢多样性逐渐恢复平衡,但两种汤药的调控作用不尽相同。七味白术散超微饮片1/2剂量对肠道厌氧微生物代谢多样性的调控作用优于传统饮片。

Compound traditional Chinese medicine is one of the medication characteristics of traditional Chinese medicine, as well as its essence. Intestinal microbiotia have a crucial effect on hosts＇ metabolic function and life activities. Intestinal microbiotia are devoted to dissolution and transformation of the Chinese herbal medicinal ingredients by hydrolysis and restoring reaction. In order to investigate the microecological mechanisms for ultra-micro Qiweibaizhusan on diarrheal mice with intestinal dysbacteriosis, the mouse model with dyshacteriosis was constructed by intragastric administration with antibiotics mixture composed of cefradine capsules and gentamycin sulfate injection. One group of diarrheal mice were treated with the traditional decoction of Qiweibaizhusan, the other group of diarrheal mice were given 1/2 dose of ultra- micro powder Qiweibaishusan, and the normal group and the diarrheal group mice were treated with sterilized water by intragastric administration. After three days＇ treatment, the intestinal microbiotia were extracted, and their carbon metabolisms were determined by Biolog Microbial Identification System. The results showed that all groups of average of wall color development （AWCD） tended to be stable throughout 72 h cultivation period, but AWCD of the 1/2 dose of ultramicro powder group 〉 the normal group 〉 the traditional decoction group 〉 the model group. The microbial diversity index, including Shannon index and Simpson index, in the normal group were the largest, and there were significant differences compared with that in other groups （P〈0.01）. The Shannon evenness and Mclntosh index of the 1/2 dose of ultra-micro powder treated group were very significantly different compared to that of other group （P〈0.01, P〈0.05）, except that the Shannon evenness wasn＇t significantly different compared with that of the normal group （P〉0.05）. The results of clustering analysis and principal components analysis suggest that bacteriostasis function of antibiotics was prominent, and some metabolic pathways of intestinal microbiotia in mice were influenced. The metabolic diversity of intestinal microbiotia in mice was gradually regained by the regulatory functions of 1/2 dose of ultra-micro powder and traditional decoction of Qiweibaishusan. There were differences in the regulatory roles of the traditional decoction and ultra-micro powder decoction. The regulatory role of 1/2 dose of ultra-micro powder was more notable than that of traditional decoction. The ultra-micro Qiweibaizhusan had a prominent regulatory role on metabolic diversity of intestinal anaerobic microbiotia in mice, and had a good treatment effect on diarrhea with intestinal dysbacteriosis.