α7nAChR在氯化甲基汞神经毒性中的作用

The Effects of α7nAChR on the Neurotoxicity of Methylmercury Chloride

为探讨环境染污染物氯化甲基汞(methylmercury chloride,Me Hg Cl)的神经毒性作用机制,采用MTT、免疫细胞荧光、dot Blot、qRT-PCR等实验技术检测Me Hg Cl对PC12细胞活性及其α7亚型烟碱型胆碱能受体(α7 nicotinic acetylcholine receptor,α7n AChR)蛋白和mRNA表达水平的影响。结果显示Me Hg Cl抑制PC12细胞活性,显著降低α7n AChR的蛋白和mRNA的表达水平,呈浓度依赖性。上述结果表明,Me Hg Cl对神经细胞毒性作用可能与抑制α7n AChR表达有关。

To investigate the neurotoxicity of environmental pollutant, methylmercury chloride （MeHgC1） on the nervous system, the PC 12 cell viability was detected by MTT assay. The gene expression level of α7 nicotinic ace- tylcholine receptor, α7nAChR, was evaluated by inamunofluorescence, Dot-Blot and qRT-PCR both at protein and mRNA levels when PC12 cells were treated with MeHgC1. The results demonstrated that MeHgC1 impaired the cell viability and inhibited α7nAChR gene expression both in transcriptional and translational levels in a dose-depend- ent manner. These results illustrated that the neurotoxicity of MeHgC1 might be associated with the inhibition of α7nAChR gene expression.