苯并[a]芘和菲对缢蛏血细胞DNA损伤的研究

Effects of Benzo[a]pyrene and Phenanthrene on DNA Damage of Hemolymph Cell in Sinonovacula constricta

为研究苯并[a]芘和菲对缢蛏的毒性效应,将缢蛏(Sinonovacula constricta)分别暴露于浓度为0.45 mg·L-1、0.15 mg·L-1、0.05 mg·L-1苯并[a]芘溶液和0.45 mg·L-1、0.15 mg·L-1、0.05 mg·L-1菲的溶液中,采用单细胞凝胶电泳实验(彗星实验)技术检测不同暴露时间缢蛏血淋巴细胞的DNA损伤程度,对照组为清洁海水。结果显示,高浓度(0.45 mg·L-1)苯并[a]芘溶液和(0.45 mg·L-1)菲溶液在短期(7 d)内即可导致缢蛏血细胞显著的DNA损伤,并且随苯并芘[a]和菲浓度的增大和暴露时间的延长,DNA损伤程度增加。21 d恢复实验后,各浓度组DNA损伤又均有不同程度的恢复,但中高浓度组(0.45 mg·L-1和0.15 mg·L-1)与对照组仍显著性差异。两种多环芳烃物质对缢蛏血细胞的DNA损伤作用均存在较显著时间-剂量-效应关系。其中,苯并芘[a]对缢蛏血细胞的DNA损伤作用要高于菲。

The paper reported the DNA damage effect of Benzo[a]pyrene（BaP） and phenanthrene （PHE） on the calm Sinonovacula constricta under different concentration conditions with the single cell microgel electrophoresis （SCGE） technique. The calm were treated with concentrations （BaP： 0.45, 0.15, 0.05 mg· L-1 and PHE： 0.45, 0.15, 0.05 mg· L-1） in control group was 0.00 mg· L-1 for 15 days and were removed pollution for 6 days. The result showed the tail DNA percentage （DNA%） and TL/D of the 0.45 mg· L-1 groups were significantly different from the control group （P〈0.05） alter 7 days exposure period. Furthermore, the damage intensity of treated groups increased gradually with respect to the increasing of Bap and PHE. Under the same concentration, the tail DNA% and TL/D increased gradually with the timeof exposure. The DNA damage induced by BaP and PHE was very serious under the concentration of 0.45 mg. L-1. There was significant time-nd dose-dependent damage to the hemolymph cells. In addition, the toxic effect of BaP was much more than PHE. The comet assay was proven to be a useful tool for detecting DNA damage induced by PAH in marine hemolymph cells.DNA damage in hemolymph cells of could indicate the effects of BaP and PHE in marine envi- ronment as a good biomarker to be used in early warning and monitoring.