纳米碳黑与重金属对BEAS-2B细胞的联合毒性作用模式评价

Evaluation of Combined Effects of NPCB and Heavy Metals on BEAS-2B Cells

通过研究空气颗粒物的代表性组分纳米碳黑(nano particle carbon black,NPCB)与重金属(Pb/Cr/Cd)联合染毒对BEAS-2B细胞存活率和LDH漏出率的影响,旨在阐明NPCB与重金属对细胞毒性的联合作用模式。检测NPCB与重金属(Pb/Cr/Cd)联合染毒24 h后BEAS-2B细胞存活率(CCK-8法)和LDH漏出率(LDH活性比色法)的变化,采用析因方差分析判断其是否存在联合毒性作用及联合作用模式。NPCB与重金属(Pb/Cr/Cd)联合染毒在细胞存活率和LDH漏出方面存在联合作用;与对照组和单独染毒组相比,低剂量Pb(125μmol·L-1)与NPCB联合染毒对细胞存活率无交互作用,对LDH漏出表现为拮抗作用;高剂量Pb(1 000μmol·L-1)与NPCB联合染毒对细胞存活率表现为协同作用,对LDH漏出无交互作用;Cr和Cd与NPCB联合染毒在细胞存活率方面均表现为协同作用;低剂量Cr和Cd与NPCB联合染毒在LDH漏出方面无交互作用,高剂量时表现为协同作用。NPCB与重金属存在联合作用,金属不同、剂量不同以及评价指标不同,其联合作用模式不尽相同。

NPCB and heavy metals are representative components of airborne particulate matter. In the present study, we investigated the combined effects of NPCB with Pb/Cr/Cd on the viability and LDH leakage of BEAS-2B Cells. After co-exposure to NPCB and heavy metals （Pb/Cr/Cd） for 24 h, cell viability and LDH leakage were detec- ted by Cell Counting Kit-8（CCK-8） and LDH Cytotoxicity Assay Kit, respectively. The types of combined effects were determined by factorial design analysis of combined effects on cell viability and LDH leakage. Compared to theNPCB and heavy metals （Pb/Cr/Cd） had control group/NPCB group and the Pb group, co-exposure to low dose of Pb （125 μmol·U-1） and NPCB had no interaction on cell viability, but showed antagonistic joint action on LDH leakage; co-exposure to high dose of Pb （1 000 μmol·L-1） and NPCB showed a synergistic effect on cell viability, but had no interaction on LDH leakage. Co-exposure to Cr/Cd and NPCB showed a synergistic effect on cell viabil- ity; whereas co-exposure to low dose of Cr/Cd and NPCB showed no interaction on LDH leakage, and co-exposure to high dose of Cr/Cd and NPCB showed a synergistic effect on LDH leakage. NPCB and heavy metals had com- bined effects on cytotoxicity. The modes of combined effects were different to different metals, different doses and different toxic endpoints.