干式生化膜片的制备及其应用

Preparation and Application of the Dry Type Biochemical Film

建立了一种快速检测血液中二氧化碳结合力(CO2-CP)、葡萄糖(GLU)、尿酸(UA)含量的干式生化分析方法。筛选出合适的膜材料作为干片载体,制备干片,用干式生化分析仪检测干片,建立了测定血液中CO2-CP、GLU、UA含量的标准曲线,对所建立的方法进行加标回收实验、稳定性实验、批间、批内重复性实验、方法学比较。此法制备的干片颜色梯度明显、显色均匀,测定CO2-CP、GLU、UA的线性范围较宽;批间与批内变异系数均小于5%,重复性良好;与临床湿化学检测相比具有良好的相关性,相关系数大于0.99,可应用于临床检测。

A dry biochemical method for simple and rapid measurement of carbon dioxide-combining capacity, glucose and uric acid in the blood was developed. Appropriate polymeric membrane materials were selected as dry slide carrier. Standard curves were established for the quantization of carbon dioxide-combining capacity, glucose and uric acid by using the dry biochemical analyzer. The dry biochemical analysis method was evaluated by linear test, stability experiment, repetitive experiment and the comparison with wet chemistry method. The three dry slides prepared above have obvious color gradient and color uniformity. The linear range of dry biochemical method established is wide. This method also has a good repeatability as its interassay and intraassay coefficient of variation are all less than 5%. The dry chemistry method and wet chemistry method shows good correlation with the correlation coefficient more than 0. 99. The dry slide can be applied to the clinical detection.