肝素结合表皮生长因子对肝癌细胞迁移、侵袭以及上皮间质转化的影响

THE EFFECTS OF HEPARIN-BINDING EGF-LIKE GROWTH FACTOR ON MIGRATION,INVASION AND EPITHELIAL-MESENCHYMAL TRANSTIONOF IN HUMAN HEPATOMA HUH7 CELLS

目的:探讨肝素结合表皮生长因子(HB-EGF)对肝癌细胞转移能力的影响。方法:划痕法观察HB-EGF对肝癌Huh7细胞的迁移能力的影响,Transwell侵袭实验检测HB-EGF作用48h后肝癌Huh7细胞的侵袭情况,RT-PCR检测上皮间质转化(EMT)分子标记物基因表达。结果:10μg/L的HB-EGF可以促进肝癌Huh7细胞的迁移,1μg/L和10μg/L的HB-EGF均可以促进肝癌Huh7细胞的侵袭。并且HB-EGF可以抑制E-钙黏蛋白表达同时促进Slug的表达。结论:HB-EGF可以促进肝癌Huh7细胞的迁移、侵袭以及EMT。

Objective： To investigate the effects of Heparin-binding EGF-like growth factor （HB-EGF） on the metastatic ability of HCC cell. Methods： The effects of HB-EGF on the migration ability of Huh7 cell was tested by a scrape wound healing and the invasion ability of Huh7 after treatment with recombinant human HB-EGF for 48 h was tested by transwell chambers coated with matrix gel. And the relative markers of EMT were detected by RT-PCR. Results： 10 μg/L HB-EGF could induce migration of Hun7 cell. Meanwhile,1 μg/L and 10 μg/L HB-EGF could promote invasion of Huh7 cell. The results of RT-PCR showed that HB-EFG could inhibit E-cadherin expression and improve the expression of Slug. Conclusion： HB-EGF not only can promote the ability of invasion and metastasis of Huh7,but also influence on the EMT.