肝癌患者高表达神经细胞黏附分子L1预后差

High level of L1CAM predicting poor prognosis in hepatocellular carcinoma patients

目的神经细胞黏附分子L1(L1CAM)在肝癌中的表达及其与临床预后的关系。方法对110例肝癌样本用Western blot及qRT-PCR方法检测L1CAM蛋白和mRNA的表达,分析了L1CAM表达与肝癌患者总体生存率的关系。结果肝癌组织L1CAM蛋白、mRNA表达水平显著高于对应非肿瘤组织(P<0.01);在中-低分化肝癌患者L1CAM表达量高于高分化肝癌患者(P<0.01);L1CAM mRNA的表达与肿瘤的分化程度、TNM分期密切相关(P<0.05);采用Kaplan-Meier法生存分析,高表达L1CAM患者预后差(P<0.01)。结论 L1CAM与肝癌的发生、发展具有相关性,或许可作为评估肝癌患者预后的指标之一。

Objective To investigate the clinical significance and prognostic value of PKD3 expression in human hepatocellu- lar carcinoma （HCC） after hepatectomy. Methods We analyzed mRNA expression of L1CAM in 110 HCCs by quantitative real- time PCR （qRT-PCR） and western blot,and the relationship among the overall survival of HCCs. Results The relative protein and mRNA expression level of L1CAM was up-regulated in HCCs comparing with adjacent non tumor liver tissues （P〈0.01）. L1CAM expression in the well-differentiated group was higher than that in the poor-differentiated group （P〈0.01）. The expression of L1CAM mRNA was significantly correlated with tumor differentiation and TNM stage （P〈0.05）. The prognosis of patients with high expression L1CAM was poor （P〈0.01）. Conclusion L1CAM expression is related to occurrence and development of HCCs and may predict the prognosis of HCCs after hepatectomy.