大鼠体内黄芩苷分析方法的建立

ESTABLISHMENT OF ANALYSIS METHOD OF BAICALIN IN RATS

目的:建立黄芩苷在SD大鼠体内的分析方法。方法:采用高效液相色谱法,使用色谱柱为ODS C18色谱柱(4.6mm×250mm,5μm),流动相为乙腈:2%冰醋酸=20:80,流速为1.0ml/min,检测波长为278nm,柱温35℃,对黄芩苷对照品及模拟生物样品进行测定。结果:黄芩苷的浓度在0.015μg/ml-37.5μg/ml范围线性关系良好(r=0.9996),高(37.5μg/ml)、中(7.5μg/ml)、低(0.75μg/ml)三个模拟生物样品的平均提取回收率分别为90%、96.12%、99.91%。结论:本研究建立的黄芩苷在大鼠体内的分析方法简便快捷、专属性好,精密度、准确度、重复性、稳定性和提取回收率等各项分析指标能够达到生物样品中药物定量分析研究的方法学要求。

Objective： To establish analysis method of the baicalin in SD rats. Methods： Baicalin reference sub stance and simulated biological sample were determined by HPLC with ODS C18 chromatographic column（4.6mm×250mm, 5μm）： mobile phase was acetonitrile glacial acetic acid-2%（20：80, v/v）, flow rate was 1.0ml/min, detecting wave was 278 nm, column temperature was 35℃.Results： The linearity range of baic alin was 0.015μg/ml-37.5μg/ml（r =0.9996）. The average extraction recovery rate of high（37.5μg/ml）, medium（7.5μg/ml） and low（0.75μg/ml） sample were respectively 90%, 96.12% and 99.91%. Conclusions： The analysis method in this study is simple, efficient and specific; precision, accuracy, repeatability, stability and extraction recovery rate are all accord with the demands for quantitative drugs analysis.