摘要
目的:观察丝胶对2型糖尿病大鼠肾脏c-fos表达的影响。方法:48只雄性SD大鼠随机分为正常对照组、糖尿病模型组、丝胶治疗组和阳性对照组,每组12只。采用链脲佐菌素腹腔注射的方法建立2型糖尿病大鼠模型并给予丝胶(2.4g/kg/d)灌胃35天。分别采用Western Blot法和RT-PCR法检测各组大鼠肾脏c-fos蛋白和m RNA的表达。结果:糖尿病模型组大鼠肾脏c-fos蛋白及m RNA的表达较正常对照组大鼠明显升高(P<0.01);丝胶治疗组、阳性对照组大鼠肾脏c-fos蛋白及m RNA的表达较糖尿病模型组大鼠明显降低(P<0.01,P<0.05)。结论:丝胶可能通过下调肾脏c-fos的表达发挥对糖尿病时肾脏损伤的保护作用。
Objective： To observe the effects of sericin on expression of c-fos in kidney of rats with type 2 diabetes mellitus. Methods： 48 male SD rats were randomly divided into normal control group, diabetes mellitus model group, sericin treatment group and positive control group with 12 rats in each group. The type 2 diabetes rats model was made by continuously injecting streptozotocin into peritoneal cavity and then the rats were lavaged with sericin（2.4g/kg/d） for 35 days. Western Blot and RT-PCR were used to detect the c-fos protein and m RNA expression in kidney. Results： Compared with rats in normal control group, the c-fos protein and m RNA expression in kidney of rats in diabetes mellitus model group increased obviously（P 〈0.01）. The c-fos protein and m RNA expression in kidney of rats in sericin treatment group and positive control group were obviously lower than that of rats in diabetes mellitus model group（P 〈0.01, P 〈0.05）. Conclusions： Sericin may protect kidney injury during diabetes mellitus by down regulating expression of c-fos in kidney.
出处
《承德医学院学报》
2015年第4期281-284,共4页
Journal of Chengde Medical University
基金
国家自然科学基金项目(81441133)
河北省自然科学基金项目(H2013406096)
河北省高校重点发展学科"人体解剖与组织胚胎学"建设项目资助
