摘要
目的:考察黄芩不同取样部位10种黄酮类成分含量的变化。方法:使用TC-C18色谱柱(4.6mm×250mm,5μm,Agilent),流动相:乙腈与0.1%冰醋酸溶液进行梯度洗脱,检测波长276nm,流速为1.0ml/min,柱温25℃,以面积归一化法对数据进行分析。结果:秋季三年生黄芩不同取样部位10种黄酮类成分的含量比例各不相同,苷类成分含量从上到下越来越低、苷元类成分含量从上到下越来越高。结论:黄芩不同取样部位黄酮类成分的含量不同,应注意保证取样的科学性。
Objective： To investigate the changes of 10 kinds flavonoids contents in different sampling parts of Scutellaria baicalensis. Methods： TC-C18 chromatographic column was used in this study： gradient elution with acetonitrile and 0.1% glacial acetic acid as mobile phase, detection wavelength was 276 nm, flow rate was 1.0ml/min, column temperature was 25℃. The data was analyzed by area normalization method. Results： The content ratio of 10 kinds flavonoids was different in different sampling parts of third-year Scutellaria baicalensis in fall： from top to bottom, glycoside gradually decreased, while aglycone gradually increased. Conclusions： The flavonoids contents are different in different sampling parts of Scutellaria baicalensis, so researchers should ensure the scientific nature of sampling.
出处
《承德医学院学报》
2015年第4期284-286,共3页
Journal of Chengde Medical University
关键词
黄芩
含量比例
面积归一化法
取样部位
Scutellaria baicalensis
Content ratio
Area normalization method
Sampling part
