Livin、VEGF在骨肉瘤组织中的表达及预后的关系

Correlation between the expression of Livin and VEGF in osteosarcoma and patient's prognosis

目的观察凋亡抑制蛋白Livin、血管内皮生长因子(VEGF)在骨肉瘤组织的表达及与预后的关系。方法采用RT-PCR和免疫组织化学SP法检测Livin、VEGF mRNA和蛋白在58例骨肉瘤和35例骨软骨瘤组织的表达。结果 Livin、VEGF mRNA在骨肉瘤组织的表达水平显著高于骨软骨瘤组织,Livin、VEGF蛋白在骨肉瘤组织的阳性表达率和表达水平显著高于骨软骨瘤组织,且二者表达与年龄、性别、肿瘤大小、病理分型、Enneking分期无关(P>0.05),而与肿瘤软组织浸润、远处转移有关(P<0.05)。Livin、VEGF表达呈显著正相关(r=0.822,P=0.001)。Livin、VEGF表达阳性的骨肉瘤患者与表达阴性者相比生存率明显降低,平均生存时间明显缩短,差异有统计学意义(P<0.05)。结论骨肉瘤组织Livin、VEGF的高表达与肿瘤浸润、转移密切相关,提示预后不良,二者可作为骨肉瘤的潜在治疗靶点及判断预后的分子指标。

Objective To investigate the correlation between expression of Livin and VEGF in osteosarcoma and patient＇s prognosis.Methods The expression levels of Livin,VEGF mRNA and proteins were detected by RT-PCR and immunohistochemistry（ SP） in 58 cases of osteosarcoma and 35 cases of osteochondroma.Results The expression levels of Livin,VEGF mRNA and proteins in osteosarcoma were significantly higher than those in osteochondroma,moreover,which were correlated to the infiltration and distant metastasis of tumor soft tissues（ P〈0.05）,however,which were not related with patient＇s age,gender,tumor size,pathological typing and Enneking staging（ P〉0.05）.The expression of Livin was positively correlated to that of VEGF in osteosarcoma（ r = 0.822,P〈0.01）.The survival rate and average survival time in patients with positive expression of Livin and VEGF were significantly lower than those of patients with negative expression of Livin and VEGF.The expression of Livin was closely correlated to that of VEGF in osteosarcoma（ r = 0.822,P〈0.01）.Conclusion The high-expression of Livin and VEGF is closely correlated with infiltration and metastasis of tumors,which suggests poor prognosis.Both of them can be regarded as the molecular indexes of treatment target and prognosis evaluation in the treatment of osteosarcoma.