含L-精氨酸库存血对大鼠红细胞的影响及对离体血管调节作用的研究

Effect of banked blood containing L-arginine on red cell and vasodilation in rats

目的探讨L-精氨酸(L-Arg)对输注库存血血管功能的调节。方法将20只SD大鼠,随机分为实验组和对照组,实验组在CPD-A保养液中加入3 000μmol/L L-Arg,对照组用等体积的0.9%氯化钠溶液作为安慰剂,分别在输注后0 d、7 d、14 d、21 d、28 d、35 d取血检测pH值、离子浓度、ME及碳酸氢盐浓度、游离血红蛋白含量、红细胞的变形性和聚集性、血气分析及游离血红蛋白含量,并对血管舒张功能进行检测。结果 2组所检测pH值、离子浓度、ME及碳酸氢盐浓度、游离血红蛋白含量、红细胞的变形性和聚集性、血气分析均差异无统计学意义(P>0.05)。实验组游离血红蛋白含量显著低于对照组,而血管舒张功能明显强于对照组。结论 L-Arg对红细胞膜具有一定的稳定作用,可以显著改善库存血的舒张血管功能。

Objective To investigate the effect of banked blood containing L-arginine（L-Arg） on red cell and vasodilation in rats.Methods Twenty SD rats were randomly divided into two groups,experimental group and control group.The 3000μmol / L L-Arg was added into CPD-A keeping liquid in experimental group,however,physiological saline was served as placebo instead in control group.On 0d,7d,14 d,21d,28 d,35d after banked blood transfusion in rats,the blood specimens were taken and pH value,the concentrations of K^＋,Na^＋,Cl,SO2,PO2,PCO2,pH,HCO3,ME,free hemoglobin,the deformability and aggregation of RBC were detected and analyzed,also the vasodilation function of rats was examined.Results There were no significant differences in pH value,ion concentration,ME,hydrocarbonate concentration,free hemoglobin contents,deformability and aggregation of red cell,blood gas analysis between the two groups（P〉0.05）.The free hemoglobin contents in experimental group were significantly lower than those in control group（P〈0.05）,however,the vasodilation function of rats in experimental group was significantly greater than that in control group（P〈0.05）.Conclusion L-Arg has certain stabilization on erythrocyte membrane,which can improve obviously vasodilation effect of banked blood without impacting the quality of banked blood by decreasing the contents of free hemoglobin during subsequent storage.