LPS直接及间接作用致小鼠急性肺损伤模型的建立

Building the acute lung injury mouse model by LPS direct and indirect challenge

目的呼吸系统的急性炎症所致的急性肺损伤(ALI)是临床常见的急症、重症,也是目前实验研究的热点。在对其发病机制和治疗手段的探讨过程中,动物模型的有效建立极其重要。方法本实验比较了气道内直接滴入和腹腔注射给入两种LPS给入方式,通过组织学病理评价、炎症细胞的细胞学反应,炎症反应细胞分泌物蛋白水平的变化等炎症反应指标,评价了LPS两种不同的给入方式对小鼠急性肺损伤(ALI)模型建立的有效性。结果组织学水平显示,气道内直接滴入LPS肺组织炎性细胞浸润的病理改变较腹腔注射LPS组更明显,肺损伤的组织学病理改变也更明显;细胞学水平,气道内直接给入LPS组肺泡灌洗液可见较多中性粒细胞渗出,而腹腔注射LPS组肺泡灌洗液中性粒细胞渗出少,与腹腔注射生理盐水组无差别;细胞因子水平,气道内直接滴入LPS肺组织组肺泡灌洗液蛋白水平明显升高,高于生理盐水对照组和腹腔注射LPS组。结论气道内直接滴入LPS是一种有效的小鼠急性肺损伤模型制备方法,可通过LPS的直接气道内分散和刺激至直接肺损伤。

Objective Acute lung injury（ALI） that results from acute inflammation of respiratory system is a popular acute and serious pulmonary symptom in clinic. The successful establishment of the mouse model for ALI is essentially important. Methods In this study, we compared the differences between injection LPS from intratrecher （i.t.） and intraperitoneal （i.p.）. By histological and pathological evaluation, cytologic reaction of inflammation cells. Change of protein secreted by inflammation cells were adopted to rate the effectiveness of model establishment induced by i.t. and i.p. injection. Results We got more obvious inflammation reaction and more efficient ALI by i.t. injection. On cytologics item, we got more exudation of neutrophil cells in BALF （bronchial alveoli lavage fluid） by i.t. injection. On cytokines item, secreted proteins were obviously increased in bronchoalveoli lavage fluid by i,t. injection. Conclusions Injection LPS by i.t. is a better method for establishment of ALI model, which leads to acute lung injury directly and immediately.