《伤寒论》泄热三方对动脉粥样硬化模型大鼠炎症的影响

The Effect of Three Prescription from Shanghanlun on the Inflammation of Atherosclerosis Model Rats

目的:观察《伤寒论》中桃核承气汤、小陷胸汤、大黄黄连泻心汤等泄热三方对动脉粥样硬化(atherosclerosis,AS)模型大鼠核转录因子κB(nuclear transcription factor-κB,NF-κB)及其所介导的炎症因子之间的影响,以探明泄热类经方对AS类疾病炎症机制的作用机理。方法:将60只SD大鼠随机分为空白对照组、桃核承气汤组、小陷胸汤组、大黄黄连泻心汤组、辛伐他汀组及模型对照组,通过高脂饲料加腹腔注射维生素D3的方法建立大鼠AS模型,从第3周开始,桃核承气汤组、小陷胸汤组、大黄黄连泻心汤组开始灌服桃核承气汤、小陷胸汤、大黄黄连泻心汤煎剂,辛伐他汀组灌服辛伐他汀水溶液,空白对照组、模型对照组分别灌服等体积的生理盐水,直到造模结束。取材后检测AS大鼠血清中氧化低密度脂蛋白(oxidized low-density lipoprotein,ox-LDL)、白细胞介素-6(interleukin-6,IL-6)、C反应蛋白(C-reactive protein,CRP)含量及主动脉中NF-κB表达,并用统计软件分析NF-κB与ox-LDL、IL-6、CRP之间的直线相关系数。结果:与病理模型组相比,桃核承气汤组、小陷胸汤组、大黄黄连泻心汤组ox-LDL、IL-6、CRP、NF-κB等指标有显著性差异(P<0.01)。经直线回归分析,各中药组NF-κB与ox-LDL、IL-6、CRP成正相关。泄热三方中,桃核承气汤组相关系数最高,说明其介导的炎症机制(抑制作用)在三组中效果最好。结论:《伤寒论》泄热三方不仅对ox-LDL、IL-6、CRP、NF-κB等指标单独有抑制作用,更能抑制NF-κB所介导的IL-6等炎症因子所诱发的炎症反应。泄热三方中,桃核承气汤组的总体治疗效果最佳。

Objective：To observe the effect of three prescriptions from Shanghanlun on the nuclear factor κB and mediated inflammatory factor of atherosclerosis model rats to prove atherosclerosis disease mechanism.Methods：Sixty SD rats were randomly divided into blank control group,Taohe Chengqi Decoction group,Xiaoxianxiong soup group,Dahuang Huanglian Xiexin Decoction group,Simvastatin group and model control group.Use the high fat diet and intraperitoneal injection of vitamin D3 method to establish the therosclerosisrat mod-el.From the third week,Taohe Chengqi Decoction group,Xiaoxianxiong soup group,Dahuang Huanglian Xiexin Decoction group were administrated with Taohe Chengqi Decoction,Xiaoxianxiong soup,Dahuang Huanglian Xiexin Decoction.The Simvastatin group were given simvastatin water solution and the blank control group,model control group were gavaged with equal volume of physiological saline to modeling the end.Detect the serum oxidized low density lipoprotein（oxidized low-density lipoprotein,ox LDL）,leukocyte mediated IL-6（IL-6）,C-reactive protein（C-reactive protein CRP）content and the aorta NF kappa B expression and the linear correlation coeffi-cient between the statistical software analysis of NF kappa B and ox LDL,IL-6 and CRP.Results：Compared the IL-6,CRP,NF kappa B of three groups,there was significant difference（P 〈0.01 ）.By linear regression analysis,the CRP-kappa B of the Chinese medicine group was positively related to ox-LDL,IL-6 and NF-κB.The correlation coefficient of Taohe Chengqi Decoction group was highest in the three groups.Conclusion：The three prescription from Shanghanlun not only have depressant effect on ox LDL,IL-6,CRP,NF kappa B and also can inhibit the NF kappa B mediated inflammatory response.Taohe Chengqi Decoction has the best effect.