五味子乙素通过p38MAPK信号通路对结肠癌SW480细胞凋亡和侵袭的影响

Influence of schisandrin B in apoptosis and invasion of SW480 cells via p38MAPK signaling pathway

目的:观察五味子乙素(SchB)对人结肠癌SW480细胞凋亡和侵袭的作用及对p38MAPK信号通路的影响,阐明其作用机制。方法:将处于对数生长期的SW480细胞分为对照组、SchB低剂量组(20μmol·L-1,SchB1组)、SchB中剂量组(40μmol·L-1,SchB2组)和SchB高剂量组(80μmol·L-1,SchB3组)。用不同剂量SchB处理SW480细胞后,MTT法检测各组细胞增殖抑制率,流式细胞术检测各组细胞凋亡率,Transwell法检测SW480细胞的侵袭情况,Western blotting法检测SW480细胞中p-p38、p38、p-p53和p53蛋白表达水平。结果:与对照组比较,SchB低、中、高剂量组SW480细胞增殖抑制率和细胞凋亡率明显升高(均P<0.01),SW480细胞的侵袭率及穿膜细胞数明显降低(P<0.01),p-p38和p-p53蛋白表达水平明显升高(P<0.05或P<0.01)。结论:SchB可抑制结肠癌SW480细胞的增殖和侵袭,促进细胞的凋亡,p38MAPK信号途径可能参与了SchB对肿瘤细胞的抑制作用。

Objective To investigate the roles of schisandrin B（SchB）on the apoptosis and invasion of human colon cancer SW480 cells and its influence in p38 MAPK signaling pathway,and to clamify the mechanisms.Methods The SW480 cells were divided into control group,low dose of SchB group（20μmol·L^-1,SchB1group）,middle dose of SchB group（40μmol·L^-1,SchB2 group）and high dose of SchB group（80μmol·L^-1,SchB3group）.After the SW480 cells were treated with different doses of SchB,the inhibitory rates of proliferation of the SW480 cells were evaluated using MTT assay,the apoptotic rates were analyzed by flow cytometry using Annexin V-FITC and PI double staining methods,the invasion activities of SW480 cells were tested using Transwell chamber,and the expression levels of p-p38,p38,p-p53 and p53 were measured by Western blotting method.Results Compared with control group,the inhibitory rates of proliferation and the apoptotic rates of colon cancer SW480 cells in SchB1,SchB2 and SchB3groups were increased（P〈0.01）,the invasion rates and the number of SW480 cells penetrating the membrane were decreased（P〈0.01）,and the expression levels of p-p38 and p-p53 proteins were increased（P〈0.05 or P〈0.01）.Conclusion SchB can inhibit the proliferation and invasion of human colon cancer SW480 cells and promote the apoptosis.p38 MAPK signaling pathway may be involved in the inhibitory effect of SchB on the tumor cells.