摘要
目的观察阿魏酸钠联合苦参素对脑缺血再灌注大鼠的脑保护作用。方法采用随机数表法将大鼠分为假手术组，模型组，依达拉奉组，阿魏酸钠组，苦参素组，联合用药低、中、高剂量组，每组12只。依达拉奉组尾静脉注射依达拉奉注射液3mg／kg；阿魏酸钠组尾静脉注射阿魏酸钠溶液25mg／kg；苦参素组尾静脉注射苦参素溶液50mg／kg；联合低、中、高剂量组分别尾静脉注射12．5mg／kg阿魏酸钠溶液联合25mg／kg苦参素溶液、25mg／kg阿魏酸钠溶液联合50mg／kg苦参素溶液、50mg／kg阿魏酸钠溶液联合100mg／kg苦参素溶液；假手术组和模型组尾静脉注射等体积生理盐水，1次／d，连续5d。除假手术组外，其余各组大鼠末次给药后30min，采用线栓法复制大鼠脑缺血再灌注模型。再灌注后24h取材，观察各组大鼠脑梗死体积、脑组织超氧化物歧化酶（SOD）、丙二醛（MDA）水平。结果阿魏酸钠、苦参素单独和联合用药组均可减少脑缺血再灌注大鼠脑梗死体积（F=73．183，P〈0．01），降低MDA水平（F=17．104，P〈0．01），提高SOD活力（F=11．417，P〈0．01），且联合用药组效果优于阿魏酸钠和苦参素单独用药组（P〈0．05）。结论阿魏酸钠和苦参素联合用药具有良好的对抗脑缺血再灌注损伤的作用，效果优于单独用药组，其机制可能与抗氧化损伤有关。
Objective To observe the protective effects of Sodium Ferulate （SF） combined with OxymaUJne （OMT） on rats cerebral ischemia-reperfusion injury （CIRI） and its mechanisms. Methods Rats were divided into a sham-operation, a model, a edaravone, a SF, a OMT and a low, a middle, a high dose of combination groups by random number table, with each group contained 12 rats. Edaravone （3 mg/kg）, SF （25 mg/kg）, OMT （50 mg/kg） and combination （SF 12.5 mg/kg ＋ OMT 25 mg/kg; SF 25 mg/kg ＋ OMT 50 mg/kg; SF 50 mg/kg ＋ OMT 100 mg/kg） were injected intravenously to the rats. Normal saline were injected to rats of the sham-operation and the model groups. One injection was operated for continuous 5 days. At 30 minutes after last administration, CIRI was made on rats except for sham-operation. At 24 hours after reperfusion, rats were sacrificed. Infarcts volumes, superoxide dismutase （SOD） and malondialdehyde （MDA） were measured. Results Infarcts volumes （F=73.183, P〈0.01） and MDA contents （F=17.104, P〈0.01） were significantly reduced and SOD vitalities （F=1 1.417,P〈0.01） was increased in the SF, OMT and the combination groups. Effects of the combination groups were significantly better than the SF or the OMT groups （P〈0.05）. Conclusion Combination of SF and OMT had good effects to treat CIRI. The mechanisms were connected with anti-oxidative damages.
出处
《国际中医中药杂志》
2015年第8期716-719,共4页
International Journal of Traditional Chinese Medicine
