南极磷虾肽对去卵巢大鼠骨质疏松症的改善作用

Ameliorative effects of peptides from Antarctic krill on osteoporosis in ovariectomized rats

目的观察南极磷虾肽(peptides from Antarctic krill,AKP)对去卵巢大鼠骨代谢、骨密度(BMD)及骨生物力学的影响,探讨其对骨质疏松症的改善作用。方法 32只雌性Wistar大鼠随机分为4组:假手术组、模型对照组、阳性对照组(阿伦磷酸钠1mg/(kg·d))和南极磷虾肽组(1 000mg/(kg·d)),大鼠摘除双侧卵巢后4周开始持续灌胃90d,分别检测血清雌二醇(E2)、血清骨代谢指标(骨源性碱性磷酸酶(BALP)、骨钙素(OCN)、I型前胶原羧基端前肽(PICP)、组织蛋白酶K(Cath-K)、I型胶原羧基端交联肽(CTX-I))和尿液中骨代谢指标(钙(Ca)、磷(P)、脱氧吡啶啉(DPD))含量,并测定大鼠骨密度和骨生物力学指标。结果南极磷虾肽能显著改善去卵巢大鼠血清中BALP、OCN和PICP的分泌(P<0.01),降低血清Cath-K、CTX-I和尿Ca、P、DPD水平(P<0.01),改善去卵巢大鼠骨代谢的高转换率;南极磷虾肽能显著增加去卵巢大鼠BMD(P<0.01),改善其骨生物力学性能(P<0.01)。结论南极磷虾肽具有改善去卵巢大鼠骨质疏松症的作用。

Objective In this study,the effects of peptides from Antarctic krill（AKP）on bone metabolism,bone mineral density（BMD）and biomechanical properties in ovariectomized rats were assessed to study their ameliorative effects of osteoporosis.Methods Thirty-two Wistar female rats were divided at random into four groups：sham-operation group,model group,positive control group（Alendronate Sodium at the dose of 1mg/（kg·d））and peptides from Antarctic krill group（1 000mg/（kg·d））.From four weeks after ovarietomy,the rats were treated with saline or tested substances respectively for 90 days.Then the serum estradiol（E2）and the main parameters of bone metabolism（bone alkaline phosphatase,osteocalein,propeptide carboxy-terminal procollagen,cathepsin-K,C-terminal cross-linked telopeptide of type I collagen,urine calcium,urine phosphorus and urine deoxypyridinoline）were measured.The bone mineral density and biomechanical properties were also tested.Results All the main parameters of bone metabolism reduced in AKP group,which meant peptides from Antarctic krill could significantly improve the metabolism of ovaritomized rats without increasing the level of estradiol.BMD of AKP group was markedly higher than that of model group.And the biomechanical properties were also improved in AKP group.Conclusion Peptides from Antarctic krill have remarkably preventive effects on osteoporosis in ovaritomized rats.