无核葡萄胚挽救试管苗驯化移栽体系优化

Optimization of acclimatization system for embryo rescue tube plantlets of seedless grape

【目的】研究炼苗移栽过程中生根粉溶液浸根时间、营养液处理、开始通风时间及遮阴处理对无核葡萄胚挽救苗炼苗及移栽的影响,探索胚挽救试管苗炼苗移栽技术。【方法】以2011年与2012年无核葡萄胚挽救杂交育种后代试管苗为材料,研究生根粉溶液浸根时间、炼苗过程中是否添加营养液、揭开保湿罩时间以及移栽大田过程中遮阴对胚挽救苗炼苗及移栽的影响。【结果】1.0mg/L生根粉溶液浸泡根系3min时植株各项生理指标较好且成活率最高;移栽过程中添加1/8 MS营养液一定程度上抑制了植株的长势;炼苗初期保湿时间为4d时炼苗成活率最高;移栽至温室或大田中时一定程度的遮阴处理有利于移栽成活率的提高与植株的生长发育。【结论】无核葡萄胚挽救试管苗炼苗前生根粉浸根处理3min、炼苗时添加1/8MS营养液、炼苗后4d开始通风以及移栽时进行遮阴能提高胚挽救试管苗的生物积累量和成活率。

[Objective] Effects of ABT soak time,nutrition,lid off period and shade treatment on em- bryo rescue seedling and transplantation were studied to improve the embryo rescue seedling transplant technique. [Method] The hybrid progeny tube plantlets of seedless grape through embryo rescue in 2011 and 2012 were selected to study the effects of ABT soak time,nutrition,lid off period and shade treatment on growth and survival rate of embryo rescue seedling in domestication. [Result] The survival rate and growth of plants were best with 1.0 mg/L ABT solution soaking for 3 min. The growth of tube plantlets was inhibited after irrigating 1/8 MS nutrient solution. Survival rate of domestication was highest with lid off 4 days after hardening. Shade treatment after transplant promoted growth and improved survival rate. [Conclusion] Bioaceumulation and survival rate of seedless grape embryo rescue tube plantlets were highest when soaking root in ABT for 3 min,adding 1/8 MS nutrient solution during hardening,and starting ventilation 4 d after hardening with shade treatment.