云南不同种源北美红杉抗寒性比较

Cold-resistance of Sequoia sempervirens from Different Provenances in Yunnan

为研究云南不同种源北美红杉的抗寒性,以生长于德钦、丽江、昆明、昌宁的北美红杉枝条为试验材料,人工设定-15℃、-20℃、-25℃、-30℃、-35℃和-40℃低温梯度处理,以4℃为对照,通过测定和分析其相对电导率、超氧化物歧化酶(SOD)、过氧化物酶(POD)活性和丙二醛(MDA)含量,结果表明,不同种源北美红杉枝条的相对电导率、SOD、POD活性、MDA含量均随着处理温度的降低而呈现上升变化,高寒地区枝条的SOD、POD活性相对较高,MDA含量和电导率较低,4个供试材料中其抗寒性强弱顺序为:德钦>丽江>昆明>昌宁。研究结果将为北美红杉跨区域引种提供参考。

The Sequoia sempervirens branches were collected from 4 different provenances （ Deqin, Lijiang, Kun- ming, and Changning） in Yunnan to examine their cold resistance. The branches were treated under 6 artificial simulated low temperatures, -15℃, -20℃, -25℃, -30℃ -35℃, and -40℃ with 4℃ as control. Relative parameters of the branches subject to cold treatment were measured, including the relative leakage of electrolytes, super oxide dismutase （ SOD ） activity, peroxidase （ POD ） activity, malondialdehyde （ MDA ）. The results showed that relative electrical conductivity, SOD, POD activity, and MDA content were changed with the temper- ature decreasing, and branches with excellent cold resistance had higher activity of SOD and POD, but lower MDA and relative leakage of electrolytes. The sequence of cold resistance was in the order of Deqin〉Lijiang〉Kunming〉 Changning. The experiment of Cold-resistance could provide reference for the introduction of Sequoia sempervirens between different regions.