纳米金对内皮细胞黏附和增殖行为的调控

Influences on Adhesion and Proliferation of Endothelial Cells with AuNPs

通过选择不同还原剂分别制备了表面Au(Ⅰ)含量为0和21.52%(原子分数)的纳米金胶体.通过内皮细胞的黏附和增殖实验,显示纳米金表面金元素价态对内皮细胞生长行为无显著影响;但游离态纳米金对内皮细胞的生长具有一定浓度依赖性的抑制作用.当纳米金的浓度从0.01 nmol/L增加到1 nmol/L时,细胞黏附率下降约16%,7 d后细胞增殖数量降低约12%.纳米金对内皮细胞的抑制效果与其固定状态有关.将纳米金共价连接在玻璃片表面后,内皮细胞在其表面的黏附及增殖效果与不含纳米金参比材料无显著性差异,黏着斑染色及AO/EB染色实验表明内皮细胞可在固定化纳米金修饰表面形成良好的黏附并在增殖过程中保持良好的活性.

In order to study the influence of endothelial cells behavior with different surface valences of gold nanoparticles（ Au NPs）,two kinds of Au NPs surfaces composed of monovalence[Au（ Ⅰ） ] or zero-valence gold[Au（ 0） ],were synthesized with different reducing agent. The cell adhesion and proliferation showed that the Au（ Ⅰ） on Au NPs will not affect the growth behavior of human umbilical vein endothelial cells（ HUVECs） compared with Au（ 0）. However,two kinds of Au NPs both inhibited the adhesion and proliferation of HUVECs with concentration dependence. When the concentration of Au NPs increasing from 0. 01 nmol / L to1 nmol / L,the adhesion rate of HUVECs decreased 16%,the number of cell proliferation after 7 d culture also decreased 12%. Further research showed that the inhibition of Au NPs with HUVECs was related to the immobilized state. After covalent modified onto the glass,the inhibition of Au NPs on HUVECs decreases significantly,the cell adhesion and proliferation on Au NPs modified surface were similar to the control. Vinculin and acridine orange / ethidium bromide（ AO / EB） stained images also illustrated that HUVECs could spread and adhere well on the immobilized Au NPs surface,and keep a good activity on the proliferation. This study would provide a reference for the Au NPs used in biomedical applications.