硫氧还原蛋白在宫颈鳞癌组织中的表达及临床意义

Expression and clinical signifi cance of sulfi redoxin expression in cervical squamous cell carcinoma tissue

目的探讨硫氧还原蛋白(Srx)在宫颈鳞癌组织中的表达及临床意义。方法采用免疫组织化学SABC法检测104例宫颈鳞癌组织及其相应癌旁组织、15例重度子宫颈上皮内瘤变(CINⅢ)组织及20例正常宫颈上皮组织中Srx的表达情况,并分析其表达情况与宫颈鳞癌临床病理特征的关系。结果 Srx在CINⅢ及宫颈鳞癌组织中的阳性率分别为73.3%(11/15)、82.7%(86/104),明显高于正常宫颈组织[35.0%(7/20)],差异有统计学意义(χ2=17.778,P=0.000)。Srx在癌组织中的表达明显高于其癌旁正常宫颈组织(χ2=56.224,P=0.000)。宫颈鳞癌组织中Srx的表达与淋巴结转移、肿瘤浸润深度及有无脉管浸润有关(P<0.05),而与患者年龄、临床分期、肿瘤大小及癌组织分化程度无关(P>0.05)。结论 Srx在宫颈鳞癌组织中高表达,有可能作为宫颈鳞癌早期诊断及预后评估的一个有价值的指标。

Objective To inquire into the expression and its clinical significance of sulfiredoxin （Srx） in cervical squamous cell carcinoma tissue. Methods SABC immunohistochemical method was used to detect the expression levels of Srx in specimens of 104 cervical squamous cell carcinoma and the corresponding adjacent tissues, 15 cervical intraepithelial neoplasm （CIN Ⅲ ）, and 20 normal cervical squamous cell epithelium tissue. The relationship between the expression of Srx protein and clinical pathological parameters of the cancer was also analyzed. Results The positive expression rates of Srx in CIN Ⅲ and cervical squamous cell carcinoma [73.3%（11/15） and 82.7%（86/104）, respectively] were significantly higher than that in normal cervical tissue [35.0%（7/20）, X^2= 17.778, P=0.000]. Meanwhile, Srx expression in cervical cancer specimens was significantly higher than that in normal adjacent tissues （X^2=56.224, P=0.000）. The positive expression of Srx in cervical squamous cell carcinoma was significantly correlated with lymph node metastasis, the depth of cancer invasion, and the infiltration of blood vessels （P〈0.05）, but it was not related with age, clinical stage （FIGO）, volume of＇primarily tumor, or degree of differentiation （P〉0.05）. Conclusion The higher expression of Srx protein might be a valuable marker for the early diagnosis and evaluation of prognosis in patients with cervical squamous cell carcinoma.