雪菊对肝癌和肺癌细胞体外抗肿瘤作用研究

Study on the Antitumor Activity in vitro of Different Extracts from Coreopsis Ticntoria Flowers of Kunlun Mountain

[目的]研究雪菊不同提取物对体外培养肿瘤细胞的抑制作用。[方法]体外培养人肝癌细胞(7404)及人非小细胞肺癌细胞(A549),用不同浓度的雪菊总黄酮纯化物、粗提物及总多糖分别对细胞作用后,MTT法检测细胞生长抑制率,流式细胞仪测细胞的凋亡率。[结果]通过MTT的方法得出雪菊不同提取物对7404和A549均有抑制作用,并呈现出浓度和时间的依赖性。流式细胞仪检测总黄酮纯化物能增强7404和A549细胞的凋亡率。[结论]雪菊不同提取物对2种癌细胞的增殖有明显抑制作用,其作用机制可能与增强细胞凋亡有关。

[ Objective ] To investigate the anti-proliferation effect of different extracts from Coreopsis Ticntoria flowers of Kunlun Mountain on the cancer cells. [ Method] The liver cancer 7404 cells and the lung cancer A549 cells were cultured in vitro. The total flavonoids and the to- tal polysaccharides of Coreopsis Ticntoria flowers interene the cells in logarithmic phase of growth. The viability of cells was measured by MTY. The cell apoptosis was detected with flow cytometry. [ Result] Different concentrations of the total flavonoids and the total polysaccharides were cytotoxic to cells in a time and dose dependent manner. The purified extracts of the totalflavonoids could enhance apoptisis rate of the cells. [ Conclusion] The anti-proliferative effect of different extracts from Coreopsis Ticntoria flowers of Kunlun Mountain on the cells was better. The mechanism may be related to the inhibition apoptisis rate of the cells.