氟比洛芬酯对坐骨神经慢性缩窄性损伤模型大鼠肌肉中P物质及神经速激肽1受体表达的影响

Effects of flurbiprofen axetil on expressions of substance P and neurokinin-1 receptor in muscle of rats with chronic constriction injury of ischiadic nerve

目的观察氟比洛芬酯对坐骨神经慢性缩窄性损伤(CCI)模型大鼠疼痛阈值及肌肉中P物质(SP)及神经速激肽1受体(NK1-R)表达的影响。方法 24只雄性SD大鼠随机分为4组,每组6只。A组:氟比洛芬酯治疗CCI大鼠(CCI大鼠暴露并结扎右侧坐骨神经,观察记录术前及术后第3、6、9、12、15日大鼠行为学改变并测定其疼痛阈值,在术后疼痛高峰期给予氟比洛芬酯5 mg/kg尾静脉注射);B组:未经治疗CCI大鼠;C组:氟比洛芬酯治疗假手术大鼠(假手术大鼠仅暴露但不结扎坐骨神经,术后给予氟比洛芬酯5 mg/kg尾静脉注射);D组:假手术大鼠。采用免疫组织化学法检测大鼠肌肉中SP及NK1-R表达。结果术后第15日,A、B组大鼠达到疼痛高峰;A组大鼠经氟比洛芬酯治疗后疼痛阈值显著升高(P<0.05);C组大鼠给予氟比洛芬酯后疼痛阈值亦升高,但差异无统计学意义(P>0.05);A组SP及NK1-R的表达呈弱阳性,B组表达为强阳性,两组差异有统计学意义(P<0.05);C、D两组表达均为弱阳性,差异无统计学意义(P>0.05)。结论氟比洛芬酯对坐骨神经CCI模型大鼠坐骨神经慢性疼痛有明显的镇痛效果,其机制可能与降低肌肉中SP及NK1-R的表达有关。

Objective To explore the effects of flurbiprofen axetil（FA） on pain threshold and expressions of substance P（SP） and neurokinin-1 receptor（NK1-R） in muscle of rats with chronic constriction injury（CCI） of ischiadic nerve.Methods Twenty-four male SD rats were randomly divided into 4 groups and each group has 6 rats.For group A,rats with CCI of ischiadic nerve were treated by FA（The right ischiadic nerve of rats was exposed and ligated.Behavior changes of rats were observed and pain threshold was measured before operation and 3,6,9,12,and 15 d after operation.Rats were injected with FA of 5 mg/kg through tail vein when pain threshold reached peak after operation）.For group B,rats with CCI of ischiadic nerve were not treated.For group C,sham operation rats were treated by FA（The ischiadic nerve of rats was exposed but was not ligated.Rats were injected with FA of 5 mg/kg through tail vein after operation）.Rats of group D were sham operation rats.Expressions of SP and NK1-R in rat muscle were detected by immunohistochemistry.Results The pain threshold of groups A and B reached peak 15 d after operation.The pain threshold of group A significantly increased after being treated by FA（P〈0.05） and the pain threshold of group C also increased after being treated by FA but the difference was not statistically significant（P〉0.05）.Expressions of SP and NK1-R of group A were weakly positive and those of group B were strongly positive,the difference between two groups was statistically significant（P〈0.05）.Expressions of SP and NK1-R of group C and group D were weakly positive and the difference was not statistically significant（P〉0.05）.Conclusion The analgesia effect of FA towards chronic pain of ischiadic nerve of rats with CCI of ischiadic nerve is obviously.The mechanism may be relevant to the decrease of expressions of SP and NK1-R in muscle.