摘要
Based on previous morphological classification and RAPD analysis, we recovered and sequenced genomic DNA of a specific fragment obtained from No. 45 Trichoderma harzianum strain in RAPD amplification. A pair of specific primers was synthesized and the RAPD markers were successfully converted into SCAR markers. The SCAR molecular marker exhibited the specificity that could differ T. harzianum from other Trichoderma strains. This pair of molecular primers was used for specific PCR amplification of 45 Trichoderma strains, and T. harzianum strains could amplify a 779 bp DNA band while other strains had no amplification products.
Based on previous morphological classification and RAPD analysis, we recovered and sequenced genomic DNA of a specific fragment obtained from No. 45 Trichoderma harzianum strain in RAPD amplification. A pair of specific primers was synthesized and the RAPD markers were successfully converted into SCAR markers. The SCAR molecular marker exhibited the specificity that could differ T. harzianum from other Trichoderma strains. This pair of molecular primers was used for specific PCR amplification of 45 Trichoderma strains, and T. harzianum strains could amplify a 779 bp DNA band while other strains had no amplification products.
基金
Supported by Natural Science Fund of Hainan Province in 2013"DNA Barcode Research of Medical Plants in Euphorbiaceae in Hainan Province"(813190)
