Novec 1230灭火剂急性吸入对小鼠血液生化指标的影响

Effects of acute inhalation of fire extinguishing agent Novec 1230 on blood biochemical indexes in mice

目的：探讨Novec 1230灭火剂急性吸入对小鼠血常规和血液生化指标的影响。方法5周龄雄性ICR小鼠120只，体质量18～22 g，按数字表法随机分为染毒组（90只）和正常对照组（对照组，30只），染毒组各30只分别暴露于5％、10％、15％浓度的Novec 1230灭火剂气体中2 h，对照组暴露于普通室内空气中，每组分别设2个时间点（染毒2 h后、染毒2 h后正常饲养14 d），每个时间点15只，检测小鼠在这2个时间点的血常规和血液生化指标。结果在5％、10％、15％浓度染毒浓度下染毒2 h后，小鼠红细胞计数（RBC）[分别为（9．99±1．16）×1012、（9．90±0．68）×1012、（8．84±1．39）×1012／L]随染毒浓度增加逐渐下降，与对照组[（9．16±1．18）×1012／L]比较差异有统计学意义（P＜0．05）；白细胞计数（WBC）[分别为（4．11±0．75）×109、（6．23±1．05）×109、（6．51±1．30）×109／L]随染毒浓度增加逐渐上升，与对照组[（5．38±1．46）×109／L]比较差异有统计学意义（P＜0．05或P＜0．01）；红细胞压积（ HCT）、红细胞平均容积（ MCV）、血小板计数（ PLT）、血红蛋白（ HGB）与对照组比较有不同程度的变化，但差异均无统计学意义（P ＞0．05）；碱性磷酸酶（ALP）[分别为（218．67±0．85）、（210．13±0．76）、（196．07±6．63）U／L]随染毒浓度增加逐渐下降，丙氨酸氨基转移酶（ALT）[分别为（51．50±1．40）、（61．47±0．76）、（62．33±11．16）U／L]、天门冬氨酸氨基转移酶（AST）[分别为（195．03±1．60）、（201．30±6．56）、（259．43±11．82）U／L]、肌酸激酶（CK）[分别为（515．50±42．95）、（834．60±76．20）、（915．63±24．18）U／L]随染毒浓度增加逐渐上升，与对照组[分别为（184．97±2．46）、（39．37±0．97）、（187．70±2．51）、（452．20±93．01）U／L]比较差异均有统计学意义（P＜0．05或P＜0．01）；饲养14 d后血常规和血液生化指标与对照组比较差异均无统计学意义（P＞0．05）。结论5％、10％、15％浓度Novec 1230灭火剂气体急性吸入，能引起小鼠感染性损伤和心肌、肝脏损伤，但在脱离染毒环境14 d后损伤即可恢复。

Objective To investigate the effects of acute inhalation of fire extinguishing agent Novec 1230 on routine blood indexes and biochemical indexes in mice .Methods One hundred and twenty male ICR mice with an age range of 5 weeks and body mass of 18-22 g were randomly divided into the normal control group and the acute inhalation group , which were further divided into 3 subgroups , each consisting of 30 animals.The animals were exposed to the fire extinguishing agent Novec 1230 at concentration levels of 5%, 10%and 15%for 2 hours respectively , while the animals in the control group were exposed to common room air.Two time points （ at 2 hours and 14 days after inhalation exposure ） were set for each subgroups , which consisted of 15 mice.The routine blood levels and serum biochemical indexes of the mice were detected at the above 2 time points.Results Following exposure to the agent at levels of 5%, 10%and 15%for 2 hours, red＆nbsp;blood cell （RBC） counts [（9.99 ±1.16） ×1012,（9.90 ±0.68） ×1012,（8.84 ±1.39） ×1012/L] decreased gradually with the increase in exposure concentrations , and statistical significance could be noted , when they were compared with those of the control group [（9.16 ±1.18）1012/L] （P0.05）.Conclusions Acute exposure of fire extinguishing agent Novec 1230 seemed to induce infectious damage , myocardial and heptal damage to mice , however , the damage afflicted on the animals seemed to recover 14 days after exposure .